Insulinotropic activity of sulfonylurea/pullulan conjugate in rat islet microcapsule.
Insulinotropic activity of sulfonylurea/pullulan conjugate in rat islet microcapsule.
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DOI：
10.1016/s0142-9612(03)00382-x
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发表时间：
2003-11
期刊：
影响因子：
14
通讯作者：
Sungwon Kim;S. Chae;K. Na;S. W. Kim;Y. Bae
中科院分区：
文献类型：
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作者：
Sungwon Kim;S. Chae;K. Na;S. W. Kim;Y. Bae
本研究采用海藻酸钠-多聚赖氨酸微囊包埋的大鼠胰岛，观察了水溶性磺酰脲/普鲁兰多糖复合物对胰岛促胰岛素活性和细胞活力的长期影响。通过将羧化格列本脲衍生物偶联到多糖普鲁兰多糖（MW= 200，000）上合成偶联物。体外静态实验表明，磺脲类药物对大鼠胰岛的刺激作用需要浓度大于50μm的微囊。在动态胰岛素分泌试验中，具有胰岛β细胞的微囊恢复了与游离胰岛相当的胰岛素分泌模式，而那些没有胰岛β细胞的微囊显示出受损的胰岛素分泌。长期（1个月）培养实验表明，微囊化胰岛的胰岛素分泌水平和对葡萄糖变化的反应能力均高于未微囊化胰岛，且形态保持良好。
The in vitro long-term effect of a water-soluble sulfonylurea/pullulan conjugate (SUP) on insulinotropic activity and cell viability was investigated using rat pancreatic islets co-entrapped with SUP in conventional alginate-poly(l-lysine) microcapsules. The conjugate was synthesized by coupling a carboxylated glibenclamide derivative to a polysaccharide, pullulan (MW=200,000). In vitro static experiment showed that sulfonylurea concentration in SUP over 50μm was required to stimulate the rat islets. In a dynamic insulin secretion test, the microcapsules of islets with SUP regained the insulin secretion pattern comparable to that of free islets, while those without SUP showed impaired insulin secretion. The long-term (1 month) culture experiment demonstrated that the microcapsules of islets with SUP, with well-preserved morphology, presented higher insulin secretion level and better ability in responding to glucose changes than those without SUP.