A new sample clean-up procedure, based on ion-pairing on RP-SPE cartridges, for the determination of ionizable pesticides
A new sample clean-up procedure, based on ion-pairing on RP-SPE cartridges, for the determination of ionizable pesticides
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DOI：
10.1080/03067310500246571
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发表时间：
2006-01
影响因子：
2.6
通讯作者：
Konstantinos P. Prousalis;Christos Kaltsonoudis;T. Tsegenidis
中科院分区：
文献类型：
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作者：
Konstantinos P. Prousalis;Christos Kaltsonoudis;T. Tsegenidis
采用反相柱固相萃取法分离柠檬提取物中的电离农药残留量。用两性激发剂，如阴离子农药十六烷基三甲基溴化铵和阳离子农药十二烷基硫酸钠对药盒进行预处理。Zwittergents和相反带电的分析物产生的离子对比本地分析物更强地保留在色谱柱上。在洗涤色谱柱后，用有机溶剂中适当浓度的洗脱液可消除干扰。最后，用乙腈洗脱农药。在此基础上，建立了柠檬中酸性农药(2，4，5-三氯苯氧基乙酸、敌敌畏和敌敌畏)和碱性农药(多菌灵和噻菌灵)残留量的分析方法。分析物在反相C18高效液相色谱柱上分离，并用UV进行检测。
Residues of ionizable pesticides in lemon extracts were isolated by a new solid-phase extraction method on reverse-phase cartridges. Cartridges were preconditioned with zwittergents such as cetyltrimethylammonium bromide for anionic pesticides and sodium dodecyl sulphate for cationic pesticides. Zwittergents and opposite charged analytes produce ion pairs that are stronger retained on the cartridge bed than the native analytes. Following washings of the cartridge, with an eluent of suitable concentration in organic solvent, resulted in interference removal. Finally, pesticides were eluted with acetonitrile. Based on the aforementioned procedure, two analytical methods were developed for the determination of acidic (2,4,5-trichlorophenoxyacetic acid, dichlorprop, and dinoseb) and basic (carbendazim and thiabendazole) pesticide residues in lemons. The analytes were separated on a reverse-phase C18 HPLC column and detected by UV.