Dentin sialoprotein in bone and dentin sialophosphoprotein gene expressed by osteoblasts
Dentin sialoprotein in bone and dentin sialophosphoprotein gene expressed by osteoblasts
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DOI：
10.1080/03008200390152296
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发表时间：
2003-01-01
影响因子：
2.9
通讯作者：
Butler, WT
中科院分区：
文献类型：
--
作者：
Qin, CL;Brunn, JC;Butler, WT
牙本质唾液蛋白（DSP）和牙本质磷蛋白（DPP）作为单一mRNA转录物表达。该转录物编码一种称为牙本质唾液磷蛋白（DSPP）的大前体蛋白。DSP、DPP和DSPP被认为是没有特异性的。近年来，我们发现dspp基因在成骨细胞和骨中均有表达。利用Western免疫印迹技术，我们在大鼠长骨Gdm/EDTA提取物中检测到DSP，其含量约为牙本质中的1/400。利用逆转录聚合酶链反应（RT-PCR）技术，对5'DSP部分（称为DSP， 1432 bp）、3'DPP序列（DPP, 2135 bp）以及覆盖DSP和DPP部分的区域（DSPP, 3471 bp）进行特异性引物，我们在MC3T3-E1细胞、ROS 17/2.8成骨样细胞和小鼠颅骨中检测了DSPP mRNA。PCR结果表明，该基因在成骨细胞中的表达水平远低于在成牙细胞中的表达水平。这些数据表明，DSPP不是牙齿特异性蛋白，并且在牙齿和骨骼中，控制DSPP表达的调节机制存在显著差异。
Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP) are expressed as a single mRNA transcript. This transcript codes for a large precursor protein termed dentin sialophosphoprotein (DSPP). DSP, DPP, and DSPP have been considered to be toot specific. Recently, we found out that the dspp gene was expressed in osteoblasts and bone. With Western immunoblots, we detected DSP in the Gdm/EDTA extracts of rat long bone, at a level of about 1/400 of that in dentin. Using reverse transcription polymerase chain reaction (RT-PCR) techniques with primers specific to the 5'DSP portion (termed DSP, 1432 bp), 3'DPP sequence (DPP, 2135 bp), and the region covering portions of both the DSP and DPP (DSPP, 3471 bp), we detected DSPP mRNA in MC3T3-E1 cells, ROS 17/2.8 osteoblast-like cells, and mouse calvaria. The results from PCR show that this gene is expressed at a much lower level in osteoblasts than in odontoblasts. The data indicate that DSPP is not a tooth-specific protein and that dramatically different regulatory mechanisms governing DSPP expression are involved in tooth and bone.