Comparison of the spectral emission of lux recombinant and bioluminescent marine bacteria.
Comparison of the spectral emission of lux recombinant and bioluminescent marine bacteria.
复制标题
DOI：
10.1002/bio.716
复制
发表时间：
2003-05
期刊：
影响因子：
--
通讯作者：
G. Thouand;P. Daniel;H. Horry;P. Picart;M. Durand;K. Killham;O. Knox;M. Dubow;M. Rousseau
中科院分区：
文献类型：
--
作者：
G. Thouand;P. Daniel;H. Horry;P. Picart;M. Durand;K. Killham;O. Knox;M. Dubow;M. Rousseau
本论文的目的是研究携带编码荧光素酶的哈氏弧菌LuxAB基因的细菌在分批培养条件下生长过程中对光谱发射的影响。比较了几个生物发光菌株的体内生物发光光谱，无论是自然发光的费氏弧菌和哈维氏弧菌，还是重组菌株(两个革兰氏阴性大肠杆菌：：LuxAB菌株和一个革兰氏阳性枯草芽孢杆菌：：LuxAB菌株)。使用最初专门用于拉曼散射的高灵敏度光谱仪记录了从400 nm到750 nm的光谱发射。用拉曼电荷耦合器件清晰地识别出两个峰，一个位于491-500 nm(+/-5 nm)，另一个位于585-595(+/-5 nm)。传统的光谱仪由于在550-650 nm范围内灵敏度低、分辨率低，用光电倍增管探测器进行光谱发射测量时只能探测到前一个峰。当比较所有被研究的细菌之间的光谱时，自然细胞和重组细胞之间、革兰氏阳性和革兰氏阴性菌株之间没有发现差异，生长条件和生长介质也没有发现改变发光光谱。
The purpose of the present paper was to study the influence of bacteria harbouring the luciferase-encoding Vibrio harveyi luxAB genes upon the spectral emission during growth in batch-culture conditions. In vivo bioluminescence spectra were compared from several bioluminescent strains, either naturally luminescent (Vibrio fischeri and Vibrio harveyi) or in recombinant strains (two Gram-negative Escherichia coli::luxAB strains and a Gram-positive Bacillus subtilis::luxAB strain). Spectral emission was recorded from 400 nm to 750 nm using a highly sensitive spectrometer initially devoted to Raman scattering. Two peaks were clearly identified, one at 491-500 nm (+/- 5 nm) and a second peak at 585-595 (+/- 5 nm) with the Raman CCD. The former peak was the only one detected with traditional spectrometers with a photomultiplier detector commonly used for spectral emission measurement, due to their lack of sensitivity and low resolution in the 550-650 nm window. When spectra were compared between all the studied bacteria, no difference was observed between natural or recombinant cells, between Gram-positive and Gram-negative strains, and growth conditions and growth medium were not found to modify the spectrum of light emission.