Evolutionary relationships in Aspergillus section Fumigata inferred from partial β-tubulin and hydrophobin DNA sequences
Evolutionary relationships in Aspergillus section Fumigata inferred from partial β-tubulin and hydrophobin DNA sequences
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DOI：
10.2307/3761325
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发表时间：
1998-09-01
期刊：
影响因子：
2.8
通讯作者：
Taylor, JW
中科院分区：
文献类型：
--
作者：
Geiser, DM;Frisvad, JC;Taylor, JW
烟曲霉是重要的动物病原菌和食品污染物。在本节中，16个目前公认的物种之间存在相当大的差异，特别是在它们的交配系统中：5个已知是严格的有丝分裂孢子，9个是同宗配合，2个是异宗配合。基于benA β-微管蛋白和rodA疏水蛋白基因的部分DNA序列，推断曲霉属烟曲霉组成员之间的系统发育关系。选择棒曲霉作为外群。这两个基因区域提供了几乎相等数量的遗传信息核苷酸字符。rodA区具有相当高的水平推断的氨基酸变异比benA区。分区同质性检验的结果表明，benA和rodA数据集没有显着的冲突，和拓扑结构的最简约的两个数据集的树不同，只有在分支，不强烈支持自举。组合数据集表明，用于分类的形态特征和次生代谢物特征与植物发生没有很强的相关性。严格有丝分裂，同宗配合（自交和异交）和异宗配合（专性异交）类群之间的混合的相互关系，表明多个独立的损失的Neutraltorya的性状态和可能的衍生同宗配合通过损失的自兼容性异宗配合。食物腐败种N. fischeri被鉴定为与最常涉及人类曲霉病的世界性物种A.烟熏。
Members of Aspergillus section Fumigati are important animal pathogens and food contaminants. There is considerable variation among the 16 currently recognized species in this section, particularly in their mating systems: five are known to be strictly mitosporic, nine are homothallic, and two are heterothallic. Phylogenetic relationships were inferred among members of Aspergillus section Fumigati based on partial DNA sequences from the benA beta-tubulin and rodA hydrophobin genes. Aspergillus clavatus was chosen as an outgroup. The two gene regions provided nearly equal numbers of phylogenetically informative nucleotide characters. The rodA region possessed a considerably higher level of inferred amino acid variation than did the benA region. The results of a partition homogeneity test showed that the benA and rodA data sets were not in significant conflict, and the topologies of the most parsimonious trees for the two data sets differed only in branches that were not strongly supported by bootstrapping. The data sets in combination showed that morphological and secondary metabolite characters used in taxonomy were not strongly correlated with phylogeny. Mixed interrelationships were found among strictly mitotic, homothallic (selfing and outcrossing) and heterothallic (obligately outcrossing) taxa, suggesting multiple independent losses of the Neosartorya sexual state and possible derivation of heterothallism from homothallism through loss of self compatibility. The food spoiling species N. fischeri was identified as the closest known meiotic relative to the cosmopolitan species most often implicated in human aspergillosis, A. fumigatus.