Contribution of upper airways to antigen-induced late airway obstructive responses in guinea pigs.
Contribution of upper airways to antigen-induced late airway obstructive responses in guinea pigs.
复制标题
上呼吸道对豚鼠抗原诱导的晚期呼吸道阻塞反应的贡献。
DOI：
10.1164/ajrccm/142.1.138
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
LemanskeJr,RF
中科院分区：
文献类型：
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作者：
Johns,K;Sorkness,R;Graziano,F;Castleman,W;LemanskeJr,RF
晚期哮喘反应的发病机制一直受到关注，因为其发展与气道阻塞、气道炎症和气道高反应性相关：慢性哮喘的所有特征。为了更精确地研究迟发反应，已经开发了许多动物模型。以前的报告描述了致敏豚鼠抗原激发后3至6和17小时气道力学的变化。然而，在这些实验中，通过鼻用雾化抗原攻击动物，并在动物通过上气道呼吸时使用全身体积描记法测量气道传导性。在受到类似挑战的大鼠中，阻力的主要立即变化发生在上呼吸道。因此，本研究的目的是评价豚鼠过敏原激发后上气道和下气道对晚期变化的贡献。远交雌性Hartley豚鼠对三种不同抗原主动或被动致敏（IgG 1抗体应答），并通过直接气管吹入（绵羊丙种球蛋白[SGG]）或恶唑酮-人血清白蛋白[OX]进行激发。HSA））或雾化（卵清蛋白10A））。通过气管切开插管在麻醉豚鼠中测量肺阻力（RL）和动态顺应性（Cdyn），并使用全身体积描记器在未麻醉的鼻呼吸豚鼠中测量比气道传导率（SGaw）。晚期应答定义为RL大于平均值+ 2 SO RL或SGaw较基线降低大于安慰剂激发（牛血清白蛋白）组的2 SO。在通过下呼吸道（SGG或OX-HSA）激发的豚鼠中，6至23小时后通过气管切开术评估RL，尽管立即出现强烈反应，但没有观察到迟发反应。
The pathogenesis of the late asthmatic response has been of interest due to the fact that its development has been associated with airway obstruction, airway inflammation, and airway hyperresponsiveness: all features of chronic asthma. In order to study more precisely late responses, a number of animal models have been developed. Previous reports have described alterations in airway mechanics at both 3 to 6 and 17 h after antigen challenge in sensitized guinea pigs. In these experiments, however, animals were challenged through the nose with aerosolized antigen, and airway conductance was measured using whole body plethysmography while the animals breathed through the upper airways. In rats similarly challenged, the predominant immediate change in resistance occurs in the upper airways. The purpose of this study, therefore, was to evaluate the contribution made by both the upper and lower airways to late changes after allergen challenge in guinea pigs. Outbred female Hartley guinea pigs were actively or passively sensitized (IgG1 antibody response) to three different antigens and challenged either by direct tracheal insufflation (sheep gamma globulin [SGG) or oxazolone-human serum albumin [OX. HSA)) or by aerosolization (ovalbumin lOA)). Lung resistance (RL) and dynamic compliance (Cdyn) were measured in anesthetized guinea pigs through a tracheostomy tube, and specific airway conductance (SGaw) was measured in unanesthetized, nose-breathing guinea pigs using a whole body plethysmograph. Late responses were defined as a RL greater than the mean+ 2 SO RL or as a decrease in SGaw from baseline greater than 2 SOfrom the placebo-challenged (bovine serum albumin) group. In guinea pigs challenged through the lower airways (SGG or OX-HSA), and in which RL was evaluated 6 to 23 h later through a tracheostomy, no late responses were observed despite vigorous immediate responses.