Comparative transcriptomes analysis of the wing disc between two silkworm strains with different size of wings.
Comparative transcriptomes analysis of the wing disc between two silkworm strains with different size of wings.
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两个不同翅尺寸家蚕品系翅盘转录组比较分析
DOI：
10.1371/journal.pone.0179560
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Li M
中科院分区：
文献类型：
--
作者：
Zhang J;Blessing D;Wu C;Liu N;Li J;Qin S;Li M
家蚕翅（B. mori）由原基发育而成，不同的B.桑品种具有不同的翅型。为了确定影响B的关键因素。在家蚕翅发育中，我们选择了分别代表正常翅和微小翅表型的菌株P50和U11。分别于游走期第1天（P50D1和U11D1）、第2天（P50D2和U11D2）和第3天（P50D3和U11D3）解剖翅盘。随后，对两种菌株进行RNA测序（RNA-Seq）以构建其基因表达谱。P50与U11差异表达的基因有628个，上调基因324个，下调基因304个。基于这些差异表达基因（DEG），通过GO富集分析确定了5个富集的基因本体（GO）术语。KEGG富集分析结果表明，DEG富集在五个途径，其中，我们确定了三个途径有关的翅膀的发展。这三条通路包括氨基糖和核苷酸糖代谢通路、蛋白酶体信号通路和Hippo信号通路。通过实时定量逆转录聚合酶链反应（qRT-PCR）进一步验证富集途径中的代表性基因。RNA-Seq和qRT-PCR结果彼此基本一致。我们的研究结果还表明，在我们的研究中获得的显着不同的基因可能参与了B的大小的发展。森翅。此外，几个KEGG富集途径可能参与调控翅形成的途径。这些结果为进一步研究B翼的发展提供了基础。森。
Wings of Bombyx mori (B. mori) develop from the primordium, and different B. mori strains have different wing types. In order to identify the key factors influencing B. mori wing development, we chose strains P50 and U11, which are typical for normal wing and minute wing phenotypes, respectively. We dissected the wing disc on the 1st-day of wandering stage (P50D1 and U11D1), 2nd-day of wandering stage (P50D2 and U11D2), and 3rd-day of wandering stage (P50D3 and U11D3). Subsequently, RNA-sequencing (RNA-Seq) was performed on both strains in order to construct their gene expression profiles. P50 exhibited 628 genes differentially expressed to U11, 324 up-regulated genes, and 304 down-regulated genes. Five enriched gene ontology (GO) terms were identified by GO enrichment analysis based on these differentially expressed genes (DEGs). KEGG enrichment analysis results showed that the DEGs were enriched in five pathways; of these, we identified three pathways related to the development of wings. The three pathways include amino sugar and nucleotide sugar metabolism pathway, proteasome signaling pathway, and the Hippo signaling pathway. The representative genes in the enrichment pathways were further verified by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The RNA-Seq and qRT-PCR results were largely consistent with each other. Our results also revealed that the significantly different genes obtained in our study might be involved in the development of the size of B. mori wings. In addition, several KEGG enriched pathways might be involved in the regulation of the pathways of wing formation. These results provide a basis for further research of wing development in B. mori.