Induction of an inhibitor of interferon action in a mouse lymphokine preparation
Induction of an inhibitor of interferon action in a mouse lymphokine preparation
复制标题
在小鼠淋巴因子制剂中诱导干扰素作用抑制剂
作者：
W. R. Fleischmann;J. Georgiades;L. Osborne;F. Dianzani;H. Johnson
在小鼠淋巴因子制剂中鉴定出干扰素作用的抑制剂。在金黄色葡萄球菌肠毒素A刺激小鼠脾细胞72 h后，首次在脾细胞上清中检测到抑制作用。在未刺激培养物的上清液中未检测到抑制剂。这种抑制剂阻断免疫和成纤维细胞干扰素的抗病毒活性。通过两步柱色谱法将抑制剂纯化1，000倍。部分纯化的抑制剂阻断高达400 U的干扰素的抗病毒活性。干扰素的免疫抑制作用也被该抑制剂阻断，提示该抑制剂可能调节干扰素的免疫调节功能。
An inhibitor of interferon action was identified in mouse lymphokine preparations. The inhibito was first detected in the supernatant fluid of mouse spleen cells at 72 h after stimulation by staphylococcal enterotoxin A. Inhibitor was not detected in supernatant fluids from unstimulated cultures. This inhibitor blocks the antiviral activity of both immune and fibroblast interferons. The inhibitor was purified 1,000-fold by two-step column chromatography. The partially purified inhibitor blocked the antiviral activity of up to 400 U of interferon. The immunosuppressive effect of interferon was also blocked by the inhibitor, suggesting that the inhibitor may modulate the immunoregulatory function of interferon.