Acute hypoxia increases S100β protein in association with blood flow redistribution away from peripheral circulations in fetal sheep
Acute hypoxia increases S100β protein in association with blood flow redistribution away from peripheral circulations in fetal sheep
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DOI：
10.1203/01.pdr.0000169999.66157.c0
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发表时间：
2005-08-01
影响因子：
3.6
通讯作者：
Gazzolo, D
中科院分区：
文献类型：
--
作者：
Giussani, DA;Thakor, AS;Gazzolo, D
我们研究了妊娠晚期胎羊急性低氧血症对胎儿血浆S100 β蛋白浓度的影响，以及这些与胎儿血流再分布和酸碱状态的关系。在全身麻醉下，5只威尔士山绵羊胎儿植入血管导管，并在股动脉和脐动脉周围植入传输时间流量传感器。手术后至少5天，胎仔接受1小时的常氧，0.5小时的低氧血症和1小时的恢复。低氧血症诱导胎儿pH（a）、动脉血氧分压、酸碱过剩和[HCO 3-]显著福尔斯，而动脉二氧化碳分压无变化。所有胎仔均出现动脉血压升高、心率下降、股血管阻力增加和脐血管阻力降低。在低氧血症期间，血浆S100 β显著升高，并保持升高直至方案结束。在个别胎儿，血浆S100 β与股血管阻力和pH值。相反，没有发现S100 β和脐血管阻力之间的关系。本研究首次报道了胎儿低氧血症伴相关酸血症的控制期导致血浆S100 β浓度持续升高，这与已知在胎儿血流再分布期间发生的血流动力学变化密切相关。这些发现为胎儿S100 β浓度的变化开辟了一个新的作用，作为围产期医学中胎儿缺氧相关酸血症的可能早期标志物。
We investigated in fetal sheep during late gestation the effects of acute hypoxemia on fetal plasma S100 beta protein concentrations and how these relate to fetal redistribution of blood flow and acid-base status. Under general anesthesia, five Welsh Mountain sheep fetuses were instrumented with vascular catheters, and transit-time flow transducers were implanted around a femoral artery and an umbilical artery. At least 5 d after surgery, fetuses were subjected to 1 h of normoxia, 0.5 h of hypoxemia, and 1 h of recovery. Hypoxemia induced significant falls in fetal pH(a), arterial oxygen pressure, acid-base excess, and [HCO3-] without alteration to arterial partial pressure of carbon dioxide. An increase in arterial blood pressure, a fall in heart rate, an increase in femoral vascular resistance, and a decrease in umbilical vascular resistance occurred in all fetuses. During hypoxemia, plasma S100 beta increased significantly and remained elevated until the end of the protocol. Within individual fetuses, plasma S100 beta correlated with femoral vascular resistance and pH. In contrast, no relationship was found between S100 beta and umbilical vascular resistance. This study reports for the first time that a controlled period of fetal hypoxemia with associated acidemia leads to persistent elevations in plasma S100 beta concentrations that strongly correlate with hemodynamic changes that are known to occur during fetal blood flow redistribution. These findings open up a new role for changes in fetal S100 beta concentrations as a possible early marker of fetal hypoxia with associated acidemia in perinatal medicine.