The ultrastructure of the accessory sex organs of the male rat. XII. Nuclear alterations of prostatic epithelial cells induced by anti-androgens.
The ultrastructure of the accessory sex organs of the male rat. XII. Nuclear alterations of prostatic epithelial cells induced by anti-androgens.
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雄性大鼠副性器官的超微结构。
DOI：
10.1007/bf00224654
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发表时间：
1976
影响因子：
3.6
通讯作者：
E. Dahl
中科院分区：
文献类型：
--
作者：
E. Dahl
对去势后2、3、5、7、21天大鼠前列腺背叶上皮细胞核的超微结构进行了观察。核仁似乎经历了进行性的解体，其正常成分部分碎裂和分散。核质的变化主要反映在染色质的凝聚上，特别是沿核膜和核仁附近。21天后，核内可见不同类型的内包涵体，核轮廓不规则，有大的凹陷。在核质内可见粗粒染色质聚集。没有观察到细胞坏死，这表明雄激素剥夺导致细胞重塑到不那么活跃的状态，细胞显著改变和分泌停止，但显然它们的一些基本功能仍然完好无损。注射睾酮完全逆转了去势诱导的改变。观察到的变化被认为是由于雄激素刺激的取消，直接影响细胞的分泌功能。这些发现支持雄激素的刺激分泌效应是通过核内雄激素受体介导的，该受体可能位于核仁相关的染色质中。也有人认为，由雄激素启动的前列腺复合体上皮细胞的分泌功能可能受核内分泌中心的调节。
The fine structure of the nuclei of epithelial cells of the dorsal lobe of the rat prostate were studied 2, 3, 5, 7 and 21 days after castration. The nucleolus appears to undergo a progressive disorganisation with partial fragmentation and dispersion of its normal components.Changes in the nucleoplasm were primarily reflected by a condensation of chromatin, particularly along the nuclear membrane and adjacent to the nucleolus. Later, different types of intranuclear inclusions were observed.After 21 days, the nuclei were characterized by an irregular outline with large indentation. Within the nucleoplasm aggregates of coarse granular chromatin were found. No cell necrosis was observed, indicating that androgen deprivation results in a remodeling of the cell to a less active state with marked cellular alterations and cessation of secretion, but apparently with some of their basic functions still intact.Injections of testosterone completely reverse the castrated-induced alterations.The changes observed are assumed to be due to the withdrawal of the androgenic stimulus, with a direct influence on the secretory function of the cell. The findings support the view that the stimulating secretory effect of androgen is mediated via an intranuclear androgen receptor, probably located in the nucleolus-associated-chromatin. It is also proposed that the secretory function of the epithelial cells of the prostatic complex, initiated by androgens, may be regulated by an intranuclear secretory center.