Activated lymphocytes increase expression of 5-lipoxygenase and its activating protein in THP-1 cells.
Activated lymphocytes increase expression of 5-lipoxygenase and its activating protein in THP-1 cells.
复制标题
活化的淋巴细胞增加 THP-1 细胞中 5-脂氧合酶及其激活蛋白的表达。
DOI：
10.1152/ajpcell.1997.273.6.c2057
复制
发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Bigby,TD
中科院分区：
文献类型：
--
作者：
Ring,WL;Riddick,CA;Baker,JR;Glass,CK;Bigby,TD
本研究的目的是探讨花生四烯酸代谢的5-脂氧合酶途径的调节淋巴细胞使用单核细胞样细胞系，THP-1。当THP-1细胞在10%凝集素激活的人淋巴细胞上清液中孵育4-7天时，它们合成5-脂氧合酶产物的能力显著增加。相反，未活化淋巴细胞的上清液没有影响。通过添加粒细胞巨噬细胞集落刺激因子（GM-CSF）或白细胞介素-3来模拟合成5-脂氧合酶产物能力的增加。这些合成能力的增加反映了酶活性的增加。增加的免疫反应性蛋白和mRNA的酶5-脂氧合酶和5-脂氧合酶激活蛋白，也发现在细胞与活化的淋巴细胞上清液条件。此外，这两种酶的mRNA的增加没有被放线菌酮阻断，这表明对稳态mRNA水平的影响不需要合成新的蛋白质。GM-CSF可复制mRNA的增加。我们的结论是，淋巴细胞可以调节5-脂氧合酶的表达在THP-1细胞在一段时间内，通过释放可溶性因子。
The aim of this study was to investigate the regulation of the 5-lipoxygenase pathway of arachidonic acid metabolism by lymphocytes using the monocyte-like cell line, THP-1. When THP-1 cells were incubated over 4–7 days in 10% supernatant from lectin-activated human lymphocytes, their capacity to synthesize 5-lipoxygenase products was significantly increased. In contrast, the supernatant from nonactivated lymphocytes had no effect. The increase in capacity to synthesize 5-lipoxygenase products was mimicked by the addition of either granulocyte macrophage colony-stimulating factor (GM-CSF) or interleukin-3. These increases in synthetic capacity reflected increased enzymatic activity. Increased immunoreactive protein and mRNA for the enzymes 5-lipoxygenase and 5-lipoxygenase-activating protein were also found in cells conditioned with activated lymphocyte supernatants. Furthermore, the increase in mRNA for both enzymes was not blocked by cycloheximide, suggesting that the effect on steady-state mRNA levels does not require the synthesis of new protein. The increase in mRNA could be reproduced by GM-CSF. We conclude that lymphocytes can regulate the expression of 5-lipoxygenase in THP-1 cells over a period of days via the release of soluble factors.