Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX
Sialyllactose-binding modified DNA aptamer bearing additional functionality by SELEX
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DOI：
10.1016/j.bmc.2003.12.009
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发表时间：
2004-03-01
影响因子：
3.5
通讯作者：
Sawai, H
中科院分区：
文献类型：
--
作者：
Masud, MM;Kuwahara, M;Sawai, H
我们生产了一种新的阳离子电荷修饰的DNA适体的唾液酸乳糖，是一个无处不在的组成部分的细胞表面负责感染的几种病毒，通过使用基于磁性粒子的SELEX方法。经过13轮筛选，我们选择了22个克隆作为唾液酸乳糖结合DNA适体组成的几个修饰的胸苷。DNA适体可以形成三向连接结构，其可能形成唾液酸乳糖的结合位点。三向连接结构包含几个修饰的胸苷，其在C5位置具有带正电荷的氨基，这可以增强对具有带负电荷的羧基的硅烷基乳糖的结合能力。适体克隆中显示最强唾液酸乳糖结合能力的适体的解离常数为4.9 μ M。(C)2003 Elsevier Ltd.保留所有权利。
We produced a novel cationic-charged modified DNA aptamer for sialyllactose that is a ubiquitous component of the cell surface responsible for the infection of several viruses by using the magnetic-particle-based SELEX method. After 13 rounds of selection we selected 22 clones as sialyllactose-binding DNA aptamers composed of several modified thymidines. The DNA aptamers could form a three-way junction structure that likely forms a binding site for siallyllactose. The three-way junction structure contains several modified thymidines bearing a positively-charged amino group at the C5 position, which could enhance the binding ability for silalyllactose which has a negatively-charged carboxyl group. The dissociation constant of the aptamer that showed the strongest sialyllactose-binding ability among the clones of the aptamers was 4.9 muM. (C) 2003 Elsevier Ltd. All rights reserved.