Chemiluminescence biosensor for hydrogen peroxide determination by immobilizing horseradish peroxidase onto PVA-co-PE nanofiber membrane
Chemiluminescence biosensor for hydrogen peroxide determination by immobilizing horseradish peroxidase onto PVA-co-PE nanofiber membrane
复制标题
将辣根过氧化物酶固定在 PVA-co-PE 纳米纤维膜上用于测定过氧化氢的化学发光生物传感器
DOI：
10.1016/j.eurpolymj.2017.04.018
复制
发表时间：
2017-06-01
影响因子：
6
通讯作者：
Wang, Dong
中科院分区：
文献类型：
--
作者：
Luo, Mengying;Wang, Wenwen;Wang, Dong
采用固相合成法对表面含有大量活性羟基的聚乙烯-聚乙烯醇共聚物（PVA-co-PE）膜进行改性。用三聚氯氰和1，3-丙二胺对直径在200 ~ 500 nm范围内的不同粒径的聚乙烯醇-聚乙烯共聚物纳米纤维进行了改性。将胺化的PVA-co-PE共混膜进行生物素化，并将辣根过氧化物酶（HRP）成功地固定在其上，通过SEM、水接触角、FTIR和XPS等手段对膜表面的形貌、亲水性和化学结构进行了研究。将该膜用于催化鲁米诺-H2 O2反应，结果表明，该膜具有较高的催化活性、高效性、灵敏度和重复使用性。结果表明，HRP固定化PVA-co-PE复合膜可作为鲁米诺-H2 O2催化反应的固体载体材料。当H_2O_2浓度低至1 × 10 ~（-15）mol/L时，相对光单位（RLU）可达5 × 10 ~（-5）。HRP固定化PVA-co-PE复合膜是一种潜在的化学发光生物传感器，可应用于生物、医学和食品等领域。
Poly(ethylene-co-polyvinyl alcohol) (PVA-co-PE) nanofiber membrane with abundant active hydroxyl groups on the surface was modified through a solid-phase synthesis process. The PVA-co-PE nanofibers with different average diameters ranging from 200 to 500 nm were modified by cyanuric chloride and 1, 3-propanediamine. The aminated PVA-co-PE nanofiber membrane was subsequently biotinylated and then horseradish peroxidase (HRP) was successfully immobilized onto it. The morphologies, hydrophilicity and chemical structures of membranes surface were investigated by SEM, water contact angle, FTIR and XPS. The HRP-immobilized PVA-co-PE nanofiber membrane revealed high activity, efficiency, sensitivity and reusability when it was used to catalyze the luminol-H2O2 reaction. The results demonstrated that the HRP-immobilized PVA-co-PE nanofiber membrane could be a promising candidate as solid support materials for catalysis of Luminol-H2O2. The relative light unit (RLU) could run up to 5 x 10(5) when the H2O2 concentration was low to 1 x 10(-15) mol/L. The HRP-immobilized PVA-co-PE nanofiber membrane was the potential chemiluminescence biosensor which could be applied in biological, medical and food fields.