p16INK4a Immunostaining Identifies Occult CIN Lesions in HPV-positive Women
p16INK4a Immunostaining Identifies Occult CIN Lesions in HPV-positive Women
复制标题
DOI：
10.1097/pgp.0b013e31817e9ac5
复制
发表时间：
2009-01-01
影响因子：
2.4
通讯作者：
Torne, Aureli
中科院分区：
文献类型：
--
作者：
Ordi, Jaume;Garcia, Sonia;Torne, Aureli
评估 p16(INK4a) 染色是否有助于识别活检阴性、高危人乳头瘤病毒 (HR-HPV) 阳性女性中被低估的宫颈上皮内瘤变 (CIN)。在 1,259 名接受组织学研究并使用 Hybrid Capture 2 测试同时进行 HR-HPV 检测的女性中，我们选择了所有 HR-HPV 检测呈阳性且活检结果为阴性的患者 (n = 139)，以及所有 HR-HPV 检测呈阴性且活检结果为 CIN 1（26 例）或 CIN 2 至 3（11 例）的女性。在剩下的 1,083 名女性中，我们随机选择了 50 例 HR-HPV 阴性且活检呈阴性的病例作为对照，以及 100 例 HR-HPV 阳性且活检为 CIN 的病例（50 CIN 1、50 CIN 2-3）。在所有病例中，均进行了 p16(INK4a) 的免疫组织化学染色并对初始活检进行了第二次评估。 139 份活检中，有 34 份 (24.5%) HR-HPV 检测呈阳性，但活检呈阴性，但 p16(INK4a) 呈阳性。重新评估后，其中 30 例 (21.6%) 被归类为携带 CIN（11 例 CIN 1、19 例 CIN 2/3）。对于 HR-HPV 载量高于 100 相对光单位的病例，重新分类为任何级别的 CIN 或 CIN 2/3 的病例数均显着更高（P
To evaluate whether p16(INK4a) staining could help to recognize underestimated cervical intraepithelial neoplasia (CIN) in women positive for high-risk human papillomavirus (HR-HPV) with negative biopsy. Out Of 1,259 women undergoing a histologic study and a simultaneous HR-HPV detection using the Hybrid Capture 2 test, we selected all patients testing positive for HR-HPV and having a negative biopsy (n = 139), as well as all women testing negative for HR-HPV With a biopsy of either CIN 1 (26 cases) or CIN 2 to 3 (11 cases). Of the remaining 1,083 women, we randomly selected for the purpose of controls, 50 cases negative for HR-HPV with negative biopsy and 100 cases positive for HR-HPV and with biopsy of CIN (50 CIN 1, 50 CIN 2-3). In all cases, immunohistochemical staining for p16(INK4a) and a second evaluation of the initial biopsy was carried out. Thirty-four out of 139 biopsies (24.5%) testing positive for HR-HPV but having a negative biopsy were positive for p16(INK4a). Thirty of these cases (21.6%) were classified as harboring a CIN (11 CIN 1, 19 CIN 2/3) after reevaluation. Both the number of cases reclassified as CIN of any grade, or as CIN 2/3, were significantly higher for cases with HR-HPV load above 100 relative light unit (P