HYDROCARBON RECOVERY AND BIOCOMPATIBILITY OF SOLVENTS FOR EXTRACTION FROM CULTURES OF BOTRYOCOCCUS-BRAUNII
HYDROCARBON RECOVERY AND BIOCOMPATIBILITY OF SOLVENTS FOR EXTRACTION FROM CULTURES OF BOTRYOCOCCUS-BRAUNII
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DOI：
10.1002/bit.260340605
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发表时间：
1989-09-01
影响因子：
3.8
通讯作者：
DAUGULIS, AJ
中科院分区：
文献类型：
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作者：
FRENZ, J;LARGEAU, C;DAUGULIS, AJ
在不同的接触条件下，测试了各种与水不混溶的溶剂与富烃微藻Botryococcus braunii的生物相容性和烃回收率。首先从1500种化合物的数据库中选择了18种溶剂（为从酵母发酵中回收乙醇而编制的溶剂选择）。这些候选溶剂中的9种被证明与B是生物相容的。brauni跟随短的接触时间。这种生物相容性往往与高分子量和高沸点有关，但强烈依赖于溶剂的化学结构。低极性对生物相容性至关重要，计算的辛醇-水分配系数或通过反相高效液相色谱法（HPLC）测定的容量因子是与B的生物相容性的合适预测因子。直接从藻类培养物中高回收烃需要相对极性的溶剂，因此不利于维持细胞活力。弱极性溶剂对细胞表面的不可及性似乎保护了藻类，但也阻止了储存在B中的烃的大量回收。布劳内外墙。为了实现高回收率，必须对通过过滤浓缩的藻类进行与溶剂的接触。然后，B的大部分。在短的接触时间后，可以回收布朗烃，而不损害细胞活力。在这些条件下，相关的溶剂性质是对非极性烃的亲和力，并且用己烷实现最高的回收率，接触30分钟后约70%。
Various water‐immiscible solvents were tested for biocompatibility and hydrocarbon recovery under different contact conditions with the hydrocarbon‐rich microalgaBotryococcus braunii.Eighteen solvents were first selected from a database of 1500 compounds (compiled for solvent selection for ethanol recovery fromSaccharomyces cerevisiaefermentation). Nine of these candidate solvents were shown to be biocompatible withB. brauniifollowing short contact times. This biocompatibility tends to be associated with high molecular weights and high boiling points but strongly depends on solvent chemical structure. A low polarity is essential to biocompatibility and calculated octanol–water partition coefficients, or capacity factors determined by reversed‐phase high‐performance liquid chromatography (HPLC), are suitable predictors of biocompatibility withB. braunii.High recoveries of hydrocarbons directly from the algal culture require relatively polar solvents and are, therefore, inimical with maintenance of cell viability. The inaccessibility of weakly polar solvents to the cell surface appears to protect the algae but also prevents substantial recovery of the hydrocarbons stored inB. brauniiouter walls. In order to achieve a high recovery, contact with the solvent must be carried out on algae concentrated by filtration. Then, a large fraction ofB. brauniihydrocarbons can be recovered, after a short contact time, without impairing cell viability. Under these conditions, the pertinent solvent property is affinity for the nonpolar hydrocarbons, and the highest recovery yield, ∼70% after contact for 30 min, is achieved with hexane.