Supported liquid membrane as a novel tool for driving the equilibrium of ω-transaminase catalyzed asymmetric synthesis
Supported liquid membrane as a novel tool for driving the equilibrium of ω-transaminase catalyzed asymmetric synthesis
复制标题
DOI：
10.1016/j.jbiotec.2014.03.022
复制
发表时间：
2014-06-10
影响因子：
4.1
通讯作者：
Grey, Carl
中科院分区：
文献类型：
--
作者：
Rehn, Gustav;Adlercreutz, Patrick;Grey, Carl
生产具有工业重要性的手性胺的一个有吸引力的选择是通过使用ω-转氨酶的不对称合成。然而，要达到高收益率，往往需要一种改变均衡地位的战略。本文介绍了一种新的策略来处理这个问题。它涉及使用支撑液膜（SLM）连同填充床反应器。该反应器含有大肠杆菌细胞与ω-转氨酶从节杆菌citreus，通过絮凝与壳聚糖固定。SLM由中空纤维膜接触器组成，其中孔包含十一烷。该系统能够连续萃取胺产物，并用于成功地移动（S）-α-甲基苄胺（MBA）的不对称合成中的平衡。达到98%的转化率，相比之下，没有产物提取的转化率为50%。此外，实现了所产生的MBA的选择性提取。80 h后，产品浓度达到55 g/l，系统显示出良好的连续操作潜力。版权所有（C）2014 Elsevier B. V.保留所有权利
An attractive option to produce chiral amines of industrial importance is through asymmetric synthesis using omega-transaminase. However, reaching high yields often requires a strategy for shifting the equilibrium position. This paper describes a novel strategy for handling this problem. It involves the use of a supported liquid membrane (SLM) together with a packed bed reactor. The reactor contains Escherichia coli cells with omega-transaminase from Arthrobacter citreus, immobilized by flocculation with chitosan. The SLM consists of a hollow fibre membrane contactor in which the pores contain undecane. The system enables continuous extraction of the amine product and was used to successfully shift the equilibrium in asymmetric synthesis of (S)-alpha-methylbenzylamine (MBA). A conversion of 98% was reached, compared to 50% without product extraction. Moreover, a selective extraction of the produced MBA was realized. A high product concentration of 55g/l was reached after 80h, and the system showed promising potential for continuous operation. Copyright (C) 2014 Elsevier B.V. All rights reserved