IN-VIVO REPRESSION BY A SITE-SPECIFIC DNA-BINDING PROTEIN DESIGNED AGAINST AN ONCOGENIC SEQUENCE
IN-VIVO REPRESSION BY A SITE-SPECIFIC DNA-BINDING PROTEIN DESIGNED AGAINST AN ONCOGENIC SEQUENCE
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DOI：
10.1038/372642a0
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发表时间：
1994-12-15
期刊：
影响因子：
64.8
通讯作者：
KLUG, A
中科院分区：
文献类型：
--
作者：
CHOO, Y;SANCHEZGARCIA, I;KLUG, A
由三个锌指组成的dna结合肽已被设计成特异性结合BCR-ABL融合癌基因的一个独特的9碱基对区域，而不是亲本基因组序列。在培养的转化细胞中，可能与染色体DNA中的靶癌基因结合，并导致转录受阻。因此，小鼠细胞由于癌基因的作用而不依赖于生长因子，在用表达肽的载体短暂转染后，细胞又恢复了对生长因子的依赖。
A DNA-binding peptide comprising three zinc-fingers has been engineered to bind specifically to a unique nine-base-pair region of a BCR-ABL fusion oncogene in preference to the parent genomic sequences. Binding to the target oncogene in chromosomal DNA is possible In transformed cells in culture, and results in blockage of transcription. Consequently, murine cells rendered independent of growth factors by the action of the oncogene revert to factor dependence upon transient transfection with a vector expressing the peptide.