The Salmonella Enteritidis TolC outer membrane channel is essential for egg white survival
The Salmonella Enteritidis TolC outer membrane channel is essential for egg white survival
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DOI：
10.3382/ps/pey584
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发表时间：
2019-05-01
期刊：
影响因子：
4.4
通讯作者：
Van Immerseel, F.
中科院分区：
文献类型：
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作者：
Raspoet, R.;Eeckhaut, V;Van Immerseel, F.
肠道沙门氏菌通过在鸡蛋白色的抗菌环境中存活而具有污染鸡蛋的潜力。由于食用了受污染的鸡蛋和鸡蛋衍生产品，这导致了人类食源性沙门氏菌感染的全球大流行。肠沙门氏菌卵白色存活的分子机制尚不完全清楚。使用体内表达技术和启动子-报告基因融合，我们表明，tolC基因的启动子，编码的TolC外膜通道，使用多药外排泵输出有害分子和分泌细菌产物，激活鸡蛋白色在鸡体温。使用沙门氏菌肠毒素tolC缺失突变体，我们表明，TolC在鸡蛋白色的生存具有重要作用。色谱分离技术和随后的分离的鸡蛋白色级分的抗微生物活性的测试导致鉴定卵转铁蛋白作为鸡蛋白色抗微生物因子，其能够抑制tolC缺失菌株的生长，但不能抑制野生型菌株的生长。我们提供的证据表明，TolC保护肠道沙门氏菌免受卵转铁蛋白介导的生长抑制在鸡蛋白色。
Salmonella Enteritidis has developed the potential to contaminate eggs by surviving in the antimicrobial environment of the hen's egg white. This has led to a worldwide pandemic of foodborne salmonellosis infections in humans due to the consumption of contaminated eggs and egg-derived products. The molecular mechanisms of Salmonella Enteritidis egg white survival are not fully clear. Using in vivo expression technology and promoter-reporter fusions we showed that the promoter of the tolC gene, encoding the TolC outer membrane channel that is used by multidrug efflux pumps to export harmful molecules and to secrete bacterial products, is activated by egg white at the chicken body temperature. Using a Salmonella Enteritidis tolC deletion mutant we showed that TolC has an important role in egg white survival. Chromatographic separation techniques and subsequent testing of antimicrobial activities of separated egg white fractions led to the identification of ovotransferrin as the egg white antimicrobial factor which is capable of inhibiting growth of a tolC deletion strain but not the wild type strain. We provide evidence that TolC protects Salmonella Enteritidis against ovotransferrin-mediated growth inhibition in egg white.