Body weight and energy homeostasis was not affected in C57BL/6 mice fed high whey protein or leucine-supplemented low-fat diets
Body weight and energy homeostasis was not affected in C57BL/6 mice fed high whey protein or leucine-supplemented low-fat diets
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DOI：
10.1007/s00394-010-0155-2
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发表时间：
2011-09-01
影响因子：
5
通讯作者：
Klaus, Susanne
中科院分区：
文献类型：
--
作者：
Noatsch, Anne;Petzke, Klaus J.;Klaus, Susanne
亮氨酸被认为是高蛋白饮食调节途径的营养信号，与代谢综合征参数的缓解有关。然而，这个问题仍然存在争议。本研究的目的是评估和比较高蛋白饮食与饮食中补充亮氨酸对小鼠的影响，特别是对能量稳态，身体组成和解偶联蛋白（UCP）表达的影响，雄性C57 BL/6小鼠暴露于半合成饲料14周，半合成饲料含有20%（足够的蛋白质含量，AP）或50%乳清蛋白（高蛋白质含量，HP）。第三组喂食补充有L-亮氨酸（AP + L）的AP饮食，对应于HP饮食的亮氨酸含量。总脂肪含量为5%（w/w）。各组间的体重增加、体成分、能量消耗以及棕色脂肪组织中UCP 1和骨骼肌中UCP 3的蛋白表达均无差异。在HP喂养的小鼠中，与AP和AP + L相比，在葡萄糖耐量试验期间检测到血糖水平更强的增加，而血浆胰岛素在所有组中相似。补充亮氨酸不影响糖耐量。与AP相比，HP和AP + L的血浆胆固醇显著降低。与AP + L和AP组相比，HP喂养小鼠的血浆甘油三酯浓度增加了两倍。所有组的肝脏和骨骼肌甘油三酯和糖原浓度相似。HP和AP + L小鼠吸收后血浆中支链氨基酸浓度与AP组相比无显著性增加，而赖氨酸浓度则显著降低（P < 0.001）。与AP和AP + L相比，HP摄入后血浆蛋氨酸浓度较低（P < 0.05）。我们认为，与低脂饮食时的AP饮食相比，暴露于HP饮食或相应的亮氨酸补充对能量稳态和UCP表达没有显著影响。高质量乳清蛋白的使用至少可以部分解释所获得的结果。
Leucine is suggested to act as nutrient signal of high-protein diets regulating pathways associated with an alleviation of metabolic syndrome parameters. However, the subject remains controversial.The aim of this study was to assess and to compare the effects of high-protein diets with dietary leucine supplementation in mice, particularly on energy homeostasis, body composition, and expression of uncoupling protein (UCP), which are suggested to decrease food energy efficiency.Male C57BL/6 mice were exposed for 14 weeks to semi-synthetic diets containing either 20% (adequate protein content, AP) or 50% whey protein (high-protein content, HP). A third group was fed the AP diet supplemented with L-leucine (AP + L) corresponding to the leucine content of the HP diet. The total fat content was 5% (w/w).Body weight gain, body composition, energy expenditure, and protein expression of UCP1 in brown adipose tissue, and UCP3 in skeletal muscle were not different between groups. In HP-fed mice, a stronger increase in blood glucose levels was detected during glucose tolerance tests compared to AP and AP + L, whereas plasma insulin was similar in all groups. Leucine supplementation did not affect glucose tolerance. Plasma cholesterol was significantly decreased in HP and AP + L when compared to AP. Plasma triglyceride concentrations were increased twofold in HP-fed mice when compared to AP + L and AP groups. Liver and skeletal muscle triglyceride and glycogen concentrations were similar in all groups. Postabsorptive plasma concentrations of branched-chain amino acids were not significantly increased after exposure to HP and AP + L diets, whereas those of lysine were decreased in HP and AP + L mice when compared to AP (P < 0.001). Plasma methionine concentrations were lower after HP intake when compared to AP and AP + L (P < 0.05).We suggest that an exposure of mice to HP diets or a corresponding leucine supplementation has no significant effect on energy homeostasis and UCP expression compared with AP diets when feeding a low-fat diet. The use of high-quality whey protein might at least in part explain the results obtained.