Prevalence of bacteriophages infecting Staphylococcus aureus in dairy samples and their potential as biocontrol agents
Prevalence of bacteriophages infecting Staphylococcus aureus in dairy samples and their potential as biocontrol agents
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DOI：
10.3168/jds.2008-1744
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发表时间：
2009-07-01
影响因子：
3.5
通讯作者：
Evaristo Suarez, J.
中科院分区：
文献类型：
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作者：
Garcia, P.;Madera, C.;Evaristo Suarez, J.
对乳制品样品中感染金黄色葡萄球菌的噬菌体的流行率进行了评估。十四个葡萄球菌。将金黄色葡萄球菌菌株用于75个乳制品样品的富集培养物中。所有样本均生长特异性葡萄球菌。金黄色噬菌体。根据寄主范围，共分离到8种不同的病原菌。其中三种，Phi Phi H5，Phi G7和Phi A72，在89%的样品中发现;所有分离的菌株都是温和的。噬菌体FH 5和FA 72用于针对葡萄球菌的初步细菌挑战试验。牛奶中的金黄色葡萄球菌。噬菌体混合物（1：1）比每种单一噬菌体更有效，最有可能是通过阻止溶原化细胞的存活。噬菌体抑制葡萄球菌。金黄色葡萄球菌在超高温灭菌和巴氏灭菌全脂牛奶。然而，在半脱脂原料奶中，益生菌的活性较低，在全脂原料奶中几乎没有抑制作用。杀死Staph。在室温和37 ℃下观察到金黄色葡萄球菌，但在冷藏温度下没有观察到。
The prevalence of bacteriophages infecting Staphylococcus aureus in dairy samples was assessed. Fourteen Staph. aureus strains were used in enrichment cultures of 75 dairy samples. All samples grew specific Staph. aureus bacteriophages. According to the host range, 8 different phages were isolated. Three of them, phages Phi H5, Phi G7, and Phi A72, were found in 89% of the samples; all the isolated phages were temperate. Phages FH5 and FA72 were used in preliminary bacterial challenge tests against Staph. aureus in milk. A phage mixture (1:1) was more effective than each single phage, most likely by preventing the survival of lysogenized cells. Phages inhibited Staph. aureus in UHT and pasteurized whole-fat milk. However, the phages were less active in semi-skimmed raw milk and little inhibition was achieved in whole, raw milk. Killing of Staph. aureus was observed at room temperature and at 37 degrees C, but not at refrigeration temperature.