Armadillo-related proteins promote lateral root development in Arabidopsis
Armadillo-related proteins promote lateral root development in Arabidopsis
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DOI：
10.1073/pnas.0507575103
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发表时间：
2006-01-31
影响因子：
11.1
通讯作者：
Haseloff, J
中科院分区：
文献类型：
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作者：
Coates, JC;Laplaze, L;Haseloff, J
l犰狳/β-连环蛋白和相关蛋白在动物和盘基网柄菌发育过程中具有重要功能，调节细胞分化、增殖和粘附。植物中也存在含有犰狳重复序列的蛋白质，但大多数的作用未知。与犰狳/β-连环蛋白表现出最大序列同源性的拟南芥基因称为 ARABIDILLO-1 和 -2。在这里，我们证明 ARABIDILLO-1 和 -2 促进侧根发育。 arabidillo-1/-2 突变体形成较少的侧根，而 ARABIDILLO-1 过表达品系比野生型幼苗产生更多的侧根。 ARABIDILLO-黄色荧光蛋白融合蛋白是核蛋白。 ARABIDILLO 蛋白含有 F-box 基序，因此可以靶向其他蛋白进行蛋白酶体降解。在野生型幼苗中过度表达 ARABIDILLO-1 蛋白片段（包括 F-box 片段）可将侧根形成减少至 arabidillo-1/-2 突变体的水平。我们已经证明植物 β-连环蛋白相关蛋白调节根部发育。我们认为 ARABIDILLO 蛋白可能以侧根发育抑制剂为目标进行降解，并提出拟南芥 13-连环蛋白相关蛋白定义了一条先前未表征的促进根分枝的途径。
lArmadillo/beta-catenin and related proteins have important functions during animal and Dictyostelium development, regulating cell differentiation, proliferation, and adhesion. Armadillo-repeat-containing proteins also exist in plants, but the majority have unknown roles. The Arabidopsis genes that show greatest sequence homology to Armadillo/beta-catenin are called ARABIDILLO-1 and -2. Here, we demonstrate that ARABIDILLO-1 and -2 promote lateral root development. arabidillo-1/-2 mutants form fewer lateral roots, and ARABIDILLO-1-overexpressing lines produce more lateral roots than wild-type seedlings. ARABIDILLO-yellow fluorescent protein fusions are nuclear. ARABIDILLO proteins contain an F-box motif, and thus may target other proteins for proteasomal degradation. Overexpression of ARABIDILLO-1 protein fragments, including F-box fragments, in wild-type seedlings reduces lateral root formation to the level of the arabidillo-1/-2 mutant. We have shown that plant beta-catenin-related proteins regulate root development. We suggest that ARABIDILLO proteins may target an inhibitor of lateral root development for degradation and propose that Arabidopsis 13-catenin-related proteins define a previously uncharacterized pathway that promotes root branching.