The photoactivated relaxation of smooth muscle of rabbit aorta.
The photoactivated relaxation of smooth muscle of rabbit aorta.
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DOI：
10.1085/jgp.44.3.499
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发表时间：
1961-01
影响因子：
3.8
通讯作者：
GREENBLATT, E
中科院分区：
文献类型：
--
作者：
FURCHGOTT, R F;EHRREICH, S J;GREENBLATT, E
家兔主动脉条的平滑肌，通过添加刺激药物而处于主动紧张性收缩状态，在暴露于光的过程中松弛。松弛是可逆的。标准暴露产生的松弛程度取决于暴露前主动收缩的水平，而不是用于产生收缩的刺激药物的性质。带带来的中间水平的收缩，松弛度（稳态水平）是辐射强度的矩形双曲函数。在照射过程中的松弛过程和照射后的恢复过程的动力学与以下假设一致，即初级光活化材料引发一个或多个反应，导致抑制参与主动收缩产生的某些过程的产物。光弛豫不需要氧的存在。通过降低主动脉条的温度来增强。光弛豫的作用光谱在450 mµ以上的波长处显示出相对较低的有效性。当波长低于450 mµ时，有效性显著且逐渐增加，在310 mµ时达到峰值。在280 mµ处有一个深槽。然而，峰值和谷值都可能是由于主动脉条中蛋白质的吸收而导致的内部过滤。据推测，如果可以对这种内部过滤进行校正，作用光谱将连续上升，至少低至250 mµ的波长。
Smooth muscle of strips of rabbit aorta, placed in a state of active tonic contraction by addition of a stimulating drug, relaxes during exposure to light. The relaxation is reversible. The extent of relaxation produced by a standard exposure depends on the preexposure level of active contraction but not on the nature of the stimulating drug used to produce contraction. With strips brought to an intermediate level of contraction, the degree of relaxation (steady state levels) is a rectangular hyperbolic function of radiation intensity. The kinetics of the relaxation process during irradiation and the recovery process following irradiation are consistent with the hypothesis that the primary photoactivated material initiates a reaction or reactions leading to a product which inhibits some process involved in the production of active contraction. The photorelaxation does not require the presence of oxygen. It is potentiated by reducing the temperature of the aortic strip. The action spectrum of the photorelaxation shows relatively low effectiveness at wavelengths above 450 mµ. The effectiveness increases markedly and progressively as the wavelength is lowered below 450 mµ, reaching a peak at 310 mµ. A deep trough occurs at 280 mµ. However, both peak and trough probably result from internal filtering due to absorption by proteins in the aortic strip. It is surmised that if a correction could be made for this internal filtering, the action spectrum would rise continuously down to wavelengths at least as low as 250 mµ.