DYE COUPLING AMONG IMMUNOCYTOCHEMICALLY IDENTIFIED NEURONS IN THE SUPRAOPTIC NUCLEUS - INCREASED INCIDENCE IN LACTATING RATS
DYE COUPLING AMONG IMMUNOCYTOCHEMICALLY IDENTIFIED NEURONS IN THE SUPRAOPTIC NUCLEUS - INCREASED INCIDENCE IN LACTATING RATS
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DOI：
10.1016/0306-4522(87)90047-9
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发表时间：
1987-06-01
期刊：
影响因子：
3.3
通讯作者：
COBBETT, P
中科院分区：
文献类型：
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作者：
HATTON, GI;YANG, QZ;COBBETT, P
这一假设，催产素能神经元之间的电紧张性传播有助于同步爆发在哺乳期大鼠导致的预测，催产素能神经元之间的耦合将更强，更丰富的哺乳期比非哺乳动物。我们测试了这一预测，作为一个指标的电耦合，转移神经元之间的荧光染料荧光黄CH，它跨越间隙连接。细胞内注射（共159）的染料，在视上核神经元在下丘脑切片处女雌性和哺乳期大鼠。在处女中，86次注射导致76个单个，8个耦合对和2个三联体的充满染料的神经元。相比之下，在哺乳动物中注射73次，产生51个单胎，16个成对胎和6个三胎，（增加> 100%）差异显著性P < 0.001。免疫细胞化学鉴定的染料填充的细胞显示，有一个增加的处女之间的催产素和加压素能神经元的耦合。这些结果是一致的假设，即电耦合参与同步催产素细胞爆发的哺乳动物。它们也与已发表的数据一致，表明加压素神经元在哺乳期间代谢活化（显示葡萄糖摄取增加），并可能显示相关活性。
The hypothesis that electrotonic spread among oxytoxinergic neurons contributes to synchronized bursting in the lactating rat leads to the prediction that coupling among oxytocinergic neurons would be stronger and more abundant in lactating than in non-lactating animals. We tested this prediction using, as an index of electrical coupling, transfer among neurons of the fluorescent dye Lucifer Yellow CH, which crosses gap junctions. Intracellular injections (total of 159) of the dye were made in supraoptic nucleus neurons in hypothalamic slices from virgin female and lactating rats. In virgins, 86 injections resulted in 76 single, 8 coupled pairs and 2 triplets of dye-filled neurons. In contrast, 73 injections in lactators yielded 51 single, 16 coupled pairs and 6 triplets, (> 100% increase) a difference sigificant at P < 0.001. Immunocytochemical identification of the dye-filled cells revealed that there was an increase over virgins in coupling among both oxytocinergic and vasopressinergic neurons. These results are consistent with the hypothesis that electrical coupling is involved in synchronizing oxytocin cell bursting in lactators. They are also consistent with published data indicating that vasopressin neurons are metabolically activated (show increased glucose uptake) during suckling and may show correlated activity.