Genomic diversity of 2010 Haitian cholera outbreak strains
Genomic diversity of 2010 Haitian cholera outbreak strains
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DOI：
10.1073/pnas.1207359109
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发表时间：
2012-07-17
影响因子：
11.1
通讯作者：
Colwell, Rita R.
中科院分区：
文献类型：
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作者：
Hasan, Nur A.;Choi, Seon Young;Colwell, Rita R.
过去200年中，数百万人死于霍乱，加上2010年10月以来海地霍乱的发病率和死亡率都在上升，这些都严峻地提醒人们，霍乱的病原体霍乱弧菌仍然是一种祸害。我们报告在海地霍乱流行早期从海地8个区18个城镇的受害者采集的样本中分离出霍乱弧菌O1和非O1/O139。结果表明，在霍乱流行的早期，海地同时存在两个不同的霍乱弧菌种群。由于非O1/O139型霍乱弧菌是从21%的临床标本中分离出的唯一病原体，因此不能忽视其在本次流行中的作用，无论是单独还是与O1型霍乱弧菌协同作用。采用基因组学方法研究了海地霍乱弧菌O1和非O1/O139霍乱弧菌菌株之间的异同。对从患者和环境中分离的47株O1型霍乱弧菌和29株非O1/O139型霍乱弧菌进行了测序。对海地以外同时流行的霍乱弧菌的76个基因组和8个参考菌株以及公共数据库中现有的27个霍乱弧菌基因组进行的比较基因组分析表明，霍乱弧菌具有很大的多样性，并且在其基因组内持续流动。
The millions of deaths from cholera during the past 200 y, coupled with the morbidity and mortality of cholera in Haiti since October 2010, are grim reminders that Vibrio cholerae, the etiologic agent of cholera, remains a scourge. We report the isolation of both V. cholerae O1 and non-O1/O139 early in the Haiti cholera epidemic from samples collected from victims in 18 towns across eight Arrondissements of Haiti. The results showed two distinct populations of V. cholerae coexisted in Haiti early in the epidemic. As non-O1/O139 V. cholerae was the sole pathogen isolated from 21% of the clinical specimens, its role in this epidemic, either alone or in concert with V. cholerae O1, cannot be dismissed. A genomic approach was used to examine similarities and differences among the Haitian V. cholerae O1 and V. cholerae non-O1/O139 strains. A total of 47 V. cholerae O1 and 29 V. cholerae non-O1/O139 isolates from patients and the environment were sequenced. Comparative genome analyses of the 76 genomes and eight reference strains of V. cholerae isolated in concurrent epidemics outside Haiti and 27 V. cholerae genomes available in the public database demonstrated substantial diversity of V. cholerae and ongoing flux within its genome.