PREDOMINANT OBLIGATE ANAEROBES INVADING THE DEEP LAYERS OF ROOT-CANAL DENTIN
PREDOMINANT OBLIGATE ANAEROBES INVADING THE DEEP LAYERS OF ROOT-CANAL DENTIN
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DOI：
10.1111/j.1365-2591.1990.tb00798.x
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发表时间：
1990-01-01
影响因子：
5
通讯作者：
HOSHINO, E
中科院分区：
文献类型：
--
作者：
ANDO, N;HOSHINO, E
本研究通过使用厌氧手套箱系统对八颗刚拔出的牙齿的裂面进行取样，调查侵入人类根管受感染牙本质深层（距离根管壁表面 0.5-2.0 毫米）的细菌的存在和类型。在厌氧手套箱中培养后，比在含有 30% CO2 的空气中有氧培养后回收到更多的细菌。在分离的 256 种主要细菌中，205 种（80%）是专性厌氧菌。这些发现表明，牙髓病灶深层的环境是厌氧的，有利于厌氧菌的生长。在专性厌氧菌株中，乳杆菌属（30%）和链球菌属（13%）菌株占主导地位，其次是丙酸杆菌属（9%）。没有分离到专性厌氧革兰氏阴性杆菌菌株。受感染的牙根牙本质深层的微生物群落与冠状牙本质龋损深层的微生物群落有些相似。
This study was carried out to investigate the presence and types of bacteria invading the deep layers (0.5-2.0 mm from the surface of the root canal wall) of infected dentine of human root canals by sampling with an anaerobic glove box system the split surfaces of eight freshly extracted teeth. More bacteria were recovered after incubation in an anaerobic glove box than after aerobic incubation in air with 30 per cent CO2. Out of 256 predominant bacteria isolated, 205 isolates (80 per cent) were obligate anaerobes. These findings suggest that the environment of deep layers of endodontic dentinal lesions is anaerobic and favours the growth of anaerobes. Among the obligate anaerobic isolates, strains belonging to Lactobacillus (30 per cent) and Streptococcus (13 per cent) were predominant, followed by Propionibacterium (9 per cent). No strains of obligate anaerobic Gram-negative rods were isolated. The microflora of deep layers of infected root dentine is somewhat similar to that of the deep layers of carious lesions in coronal dentine.