Telomerase inhibition promotes an initial step of cell differentiation of primate embryonic stem cell
Telomerase inhibition promotes an initial step of cell differentiation of primate embryonic stem cell
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DOI：
10.1016/j.bbrc.2011.03.044
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发表时间：
2011-04-15
影响因子：
3.1
通讯作者：
Makino, Naoki
中科院分区：
文献类型：
--
作者：
Maeda, Toyoki;Kurita, Ryo;Makino, Naoki
胚胎干细胞（Embryonic stem cell，ES细胞）是一种全能细胞，它来源于囊胚，具有分化为各种体细胞的潜能。ES细胞具有自我更新的特性和分化潜能。ES细胞具有端粒酶活性，避免端粒缩短，这是体细胞分化的特征。随着ES细胞分化的进行，它们的端粒酶活性正在丧失。然而，端粒酶活性的抑制是否促进ES细胞分化的进展还没有被说服。观察端粒酶抑制剂对绒猴ES细胞分化潜能的影响，计数端粒酶抑制剂作用下表达胚胎标志物（碱性磷酸酶和TPA-1-60）的细胞。端粒酶抑制剂对绒猴ES细胞的分化有促进作用。提示外源性抑制端粒酶活性可诱导灵长类ES细胞早期分化。(C)2011 Elsevier Inc. All rights reserved.
Embryonic stem (ES) cell is well known as a totipotent cell, which is derived from a blastcyst and has potential to differentiate into every kind of somatic cell. ES cell bears self-renewal characteristic as well as differentiation potential. ES cell bears telomerase activity to avoid telomere shortening, which is a characteristic of differentiated somatic cells. As the differentiation of ES cells proceeds, their telomerase activity is losing. However, it has not been convinced whether suppression of the telomerase activity promotes progression of ES cell differentiation. The effect of telomerase inhibitor on the differentiation potential of marmoset ES cell was assessed, counting cells expressing embryonic markers (alkaline phosphatase and TPA-1-60) under existence of a telomerase inhibitor. Telomerase inhibitor showed a promotional effect for the marmoset ES cell differentiation. This result suggests that exogenous inhibition of telomerase activity leads to induction of an early differentiation of primate ES cell. (C) 2011 Elsevier Inc. All rights reserved.