Structure and complete nucleotide sequence of the chicken alpha-smooth muscle (aortic) actin gene. An actin gene which produces multiple messenger RNAs.
Structure and complete nucleotide sequence of the chicken alpha-smooth muscle (aortic) actin gene. An actin gene which produces multiple messenger RNAs.
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发表时间：
1986-07
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通讯作者：
S. Carroll;D. Bergsma;R. Schwartz
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作者：
S. Carroll;D. Bergsma;R. Schwartz
α-平滑肌（主动脉）肌动蛋白基因是在血管平滑肌细胞中表达的肌动蛋白多基因家族的独特成员。我们已经确定了完整的核苷酸序列的11个酶对基因组DNA编码鸡α-平滑肌肌动蛋白基因。该单拷贝基因指定了一种蛋白质，其序列与牛主动脉的主要α-肌动蛋白相同。蛋白质编码序列被七个内含子中断，所述内含子位于指定氨基酸残基41/42、84/85、121/122、150、204、267和327/328的密码子处。在mRNA的5 '端非翻译区发现了第8个内含子。5 '侧翼序列含有在其它鸡肌肉肌动蛋白基因中保守的元件。基因5 '端的其它序列在至少一个人肌动蛋白基因中可以是保守的。我们已经确定了至少四个信使RNA的大小范围从约1370至2700个核苷酸（不包括聚（A）尾），这是从α-平滑肌肌动蛋白基因转录。这些RNA的3 '非翻译区的长度不同，可能是由于利用了替代的多聚腺苷酸化信号。这是第一个肌动蛋白基因的多个mRNA转录本的报告。
The alpha-smooth muscle (aortic) actin gene is a distinct member of the actin multigene family which is expressed in vascular smooth muscle cells. We have determined the complete nucleotide sequence of 11 kilobase pairs of genomic DNA encoding the chicken alpha-smooth muscle actin gene. This single copy gene specifies a protein identical in sequence to the major alpha-actin from bovine aorta. The protein-coding sequences are interrupted by seven introns which are at codons specifying amino acid residues 41/42, 84/85, 121/122, 150, 204, 267, and 327/328. An eighth intron was found in the mRNA 5' untranslated region. The 5' flanking sequences contain elements which are conserved in other chicken muscle actin genes. Additional sequences at the 5' end of the gene may be conserved in at least one human actin gene. We have identified at least four messenger RNAs ranging in size from approximately 1370 to 2700 nucleotides (excluding poly(A) tails) which are transcribed from the alpha-smooth muscle actin gene. These RNAs differ in the length of their 3' untranslated regions, probably as a result of the utilization of alternative polyadenylation signals. This is the first report of an actin gene with multiple mRNA transcripts.