A point-to-point protein-protein interaction assay reveals the signaling interplays among plant hormones and environmental cues
A point-to-point protein-protein interaction assay reveals the signaling interplays among plant hormones and environmental cues
复制标题
点对点蛋白质-蛋白质相互作用测定揭示了植物激素和环境线索之间的信号相互作用
作者：
Sun Kaiwen;Xue Xiangwen;Liu Nana;Zhu Ziqiang;Li Hong
作为固着生物，植物必须适当地协调它们的生长和发育计划与环境的变化。外源环境信号与内源植物激素反应的整合通常通过物理蛋白质-蛋白质相互作用(PPI)发生。然而，调节环境和荷尔蒙反应的全面的PPI网络尚未建立。在这项研究中，我们首先克隆了拟南芥的113个植物激素相关基因和29个光信号成分，然后使用酵母双杂交方法分别测试了它们之间的相互作用(总共2,655个测试)，最终确定了141个相互作用。基于这些相互作用的结果，我们接下来通过表征JAZ1-PYL4和JAZ1-ABI1的相互作用揭示了茉莉酸和脱落酸之间的信号串扰。因此，我们为该社区提供了一个有用的资源，用于探索植物激素和/或与光之间信号相互作用的分子机制。
As sessile organisms, plants must properly coordinate their growth and developmental programs with changes in the environment. The integration of exogenous environmental cues with endogenous plant hormone responses often occurs through physical protein–protein interactions (PPIs). However, a comprehensive PPI network that mediates environmental and hormonal responses has not been established. In this study, we initially cloned 113 phytohormone‐related genes and 29 light signaling components ofArabidopsisand then individually tested their mutual interactions (in total 2,655 tests) using a yeast‐two‐hybrid approach to ultimately identify 141 interactions. Based on these interaction results, we next revealed the signaling cross talk between jasmonate and abscisic acid by characterizing the JAZ1‐PYL4 and JAZ1‐ABI1 interactions. Thus, we generated a useful resource for the community to explore the molecular mechanisms underlying signaling interactions between plant hormones and/or with light.