Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys
Tetracycline-resistance encoding plasmids from Paenibacillus larvae, the causal agent of American foulbrood disease, isolated from commercial honeys
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DOI：
10.2436/20.1501.01.207
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发表时间：
2014-01-01
影响因子：
3.1
通讯作者：
Lopez, Ana C.
中科院分区：
文献类型：
--
作者：
Alippi, Adriana M.;Leon, Ignacio E.;Lopez, Ana C.
幼虫类芽孢杆菌是引起蜜蜂美洲幼虫病的病原体，通过携带已知四环素抗性决定簇的天然质粒获得四环素抗性。从从蜂蜜中分离的三个幼虫疟原虫四环素抗性菌株中，分离出具有几乎相同序列的5-kb环状质粒，在菌株PL 373中命名为pPL 373，在菌株PL 374中命名为pPL 374，在菌株PL 395中命名为pPL 395。这些质粒与通过滚环机制复制的小四环素编码质粒（pMA 67、pBHS 24、pBSDMV 46 A、pDMV 2、pSU 1、pAST 4和pLS 55）高度相似（99%）。核苷酸序列比较表明，pPL 373、pPL 374和pPL 395与已报道的幼虫疟原虫质粒pMA 67的差异主要在oriT区和mob基因上。这些差异表明了替代的动员和/或缀合能力。质粒pPL 373、pPL 374和pPL 395通过电穿孔分别转移，并稳定地保持在四环素敏感的P.幼虫NRRL B-14154中，它们在其中自主复制。在五种不同的革兰氏阳性菌属中存在几乎相同的质粒，即，Bhargavaea，芽孢杆菌，乳酸杆菌，类芽孢杆菌，孢子八叠球菌，栖息在不同的生态位提供了进一步的证据，四环素耐药性的遗传转移之间的环境细菌从土壤，食物，海洋栖息地和病原菌，如P.幼虫。
Paenibacillus larvae, the causal agent of American foulbrood disease in honeybees, acquires tetracycline-resistance via native plasmids carrying known tetracycline-resistance determinants. From three P. larvae tetracycline-resistant strains isolated from honeys, 5-kb-circular plasmids with almost identical sequences, designated pPL373 in strain PL373, pPL374 in strain PL374, and pPL395 in strain PL395, were isolated. These plasmids were highly similar (99%) to small tetracycline-encoding plasmids (pMA67, pBHS24, pBSDMV46A, pDMV2, pSU1, pAST4, and pLS55) that replicate by the rolling circle mechanism. Nucleotide sequences comparisons showed that pPL373, pPL374, and pPL395 mainly differed from the previously reported P. larvae plasmid pMA67 in the oriT region and mob genes. These differences suggest alternative mobilization and/or conjugation capacities. Plasmids pPL373, pPL374, and pPL395 were individually transferred by electroporation and stably maintained in tetracycline-susceptible P. larvae NRRL B-14154, in which they autonomously replicated. The presence of nearly identical plasmids in five different genera of gram-positive bacteria, i.e., Bhargavaea, Bacillus, Lactobacillus, Paenibacillus, and Sporosarcina, inhabiting diverse ecological niches provides further evidence of the genetic transfer of tetracycline resistance among environmental bacteria from soils, food, and marine habitats and from pathogenic bacteria such as P. larvae.