A determination of tumor necrosis factor expression in TMJ inflammation with the use of microarray analysis
A determination of tumor necrosis factor expression in TMJ inflammation with the use of microarray analysis
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DOI：
10.1177/154405910308201009
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发表时间：
2003-10-01
影响因子：
7.6
通讯作者：
Hutchins, B
中科院分区：
文献类型：
--
作者：
Spears, R;Oakes, R;Hutchins, B
许多不同的因素可以导致颞下颌关节组织内的炎症变化。本研究检测了TNF-α的表达。炎症过程中TMJ组织中其受体发生变化。成年雄性大鼠双侧注射完全弗氏佐剂（CFA）到颞下颌关节或作为未注射的对照，并杀死CFA治疗后两天。取颞下颌关节组织，通过基因芯片分析、RT-PCR、Western blot和ELISA检测TNF-α及其受体的表达。基因微阵列分析提供了基因表达变化的证据，特别是TNF-α和TNF-R1，而不是TNF-R2，在CFA治疗的TMJ组织中显着升高。然而，在CFA处理的TMJ组织中，TNF-α、TNF-R1和TNF-R2的蛋白水平均显著升高。这些结果表明，促炎细胞因子TNF-α可能在与抗炎药诱导的TMJ关节炎相关的炎症状态的发作中起重要作用。
Many different factors can lead to inflammatory changes within temporomandibular joint tissues. This investigation examined if the expression of TNF-alpha. and its receptors was altered in TMJ tissues during inflammation. Adult male rats were injected bilaterally with complete Freund's adjuvant (CFA) into the TMJ or served as uninjected controls and were killed two days after CFA treatment. TMJ tissues were removed, and expression of TNF-alpha and its receptors was examined via gene microarray analysis, RT-PCR, Western blot, and ELISA. Gene microarray analysis provided evidence for changes in gene expression, notably that TNF-alpha and TNF-R1, but not TNF-R2, were significantly elevated in CFA-treated TMJ tissues. However, protein levels of TNF-alpha, TNF-R1, and TNF-R2 were all significantly increased in CFA-treated TMJ tissues. These results indicate that the pro-inflammatory cytokine TNF-alpha may play a significant role in the onset of inflammatory conditions associated with adjuvant-induced arthritis of the TMJ.