Immunomodulatory mechanism of a purified polysaccharide isolated from Isaria cicadae Miquel on RAW264.7 cells via activating TLR4-MAPK-NF-κB signaling pathway
Immunomodulatory mechanism of a purified polysaccharide isolated from Isaria cicadae Miquel on RAW264.7 cells via activating TLR4-MAPK-NF-κB signaling pathway
复制标题
蝉衣纯化多糖通过激活 TLR4-MAPK-NF-kappa B 信号通路对 RAW264.7 细胞的免疫调节机制
DOI：
10.1016/j.ijbiomac.2020.09.035
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发表时间：
2020-12-01
影响因子：
8.2
通讯作者：
Zhang, Danyan
中科院分区：
文献类型：
--
作者：
Xu, Zhanchi;Lin, Ruyi;Zhang, Danyan
从蝉棒束孢（IsariacicadaeMiquel）中分离纯化的一种新型多糖（JCH-1）具有促进一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）和白细胞介素6（IL-6）分泌的免疫调节作用。但其免疫调节机制尚不清楚。本研究旨在探讨JCH-1的免疫调节机制。实验数据显示，JCH-1可增加Toll样受体4（TLR 4）的蛋白表达，促进丝裂原活化蛋白激酶（MAPK）的磷酸化，以及核因子-κ B（NF-kappa B）p65。重要的是，TLR 4抑制剂抑制JCH-1诱导的MAPK-NF-κ B信号通路的激活，从而抑制JCH-1诱导的NO、TNF-α和IL-6的分泌。这些结果表明JCH-1通过TLR 4-MAPK-NF-κ B信号通路激活RAW 264. 7细胞。(C)2020由Elsevier B. V.出版
The immunomodulatory effect of a novel purified polysaccharide (JCH-1) isolated fromIsaria cicadae Miquel had been confirmed to promote secretion of nitric oxide (NO), tumor necrosis factor-alpha (TNF-alpha) and interleukin6 (IL-6) in our previous study. However, the immunomodulatory mechanismwas still unclear. The purpose of this study was to investigate the immunomodulatory mechanism of JCH-1. Experimental data showed that JCH-1 could increase protein expression of toll-like receptor 4 (TLR4), promote the phosphorylation of mitogen-activated protein kinase (MAPK), as well as nuclear factor-kappa B (NF-kappa B) p65. Importantly, TLR4 inhibitor inhibited JCH-1-induced activation of MAPK-NF-kappa B signaling pathway, thus suppressed JCH-1-induced secretion of NO, TNF-alpha and IL-6. Collectively, these results indicated that JCH-1 actives RAW264.7 cells through TLR4-MAPK-NF-kappa B signaling pathway. (C) 2020 Published by Elsevier B.V.