Production of mevalonate by a metabolically-engineered Escherichia coli
Production of mevalonate by a metabolically-engineered Escherichia coli
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DOI：
10.1023/b:bile.0000044449.08268.7d
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发表时间：
2004-10-01
影响因子：
2.7
通讯作者：
Hashimoto, SI
中科院分区：
文献类型：
--
作者：
Tabata, K;Hashimoto, SI
Mevalonate is biosynthesized from acetyl-CoA and metabolized to isoprenoid compounds in a wide variety of organisms although certain types of prokaryotes employ another route for isoprenoid biosynthesis (the non-mevalonate pathway). To establish a fermentative process for mevalonate production, enzymes for mevalonate synthesis from Enterococcus faecalis were expressed in Escherichia coli, a non-mevalonate pathway bacterium. Mevalonate was accumulated, indicating a redirection of acetate metabolism by the expressed enzyme. The recombinant E. coli produced 47 g mevalonate l(-1) in 50 h of fed-batch cultivation in a 2 1 jar fermenter; this is the highest titer ever reported demonstrating the superiority of E. coli in its ability of acetyl-CoA supply and its inability is degrade mevalonate.
