Polarization-resolved second-harmonic generation imaging through a multimode fiber
Polarization-resolved second-harmonic generation imaging through a multimode fiber
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DOI：
10.1364/optica.430295
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发表时间：
2021-08-20
期刊：
影响因子：
10.4
通讯作者：
Tragardh, Johanna
中科院分区：
文献类型：
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作者：
Cifuentes, Angel;Pikalek, Tomas;Tragardh, Johanna
基于多模光纤的内窥镜最近已成为一种在组织中进行微创内窥镜检查的工具，其深度远远超出了多光子成像的范围。在这里，我们通过这种光纤内窥镜演示了无标记二次谐波发生（SHG）显微镜。我们同时完全控制光纤尖端的激发偏振态和光的空间分布，并用它来实现偏振分辨二次谐波成像，从而可以对胶原蛋白和肌球蛋白等结构蛋白进行成像和识别。我们使用内窥镜深度达 1 毫米对小鼠尾腱和心脏组织进行成像，证明我们可以区分这些结构蛋白。该方法有可能以最小的损伤对敏感组织中的肿瘤和纤维化状况进行即时原位诊断。 (C) 2021 年美国光学协会根据 OSA 开放获取出版协议的条款
Multimode fiber-based endoscopes have recently emerged as a tool for minimally invasive endoscopy in tissue, at depths well beyond the reach of multiphoton imaging. Here, we demonstrate label-free second-harmonic generation (SHG) microscopy through such a fiber endoscope. We simultaneously fully control the excitation polarization state and the spatial distribution of the light at the fiber tip, and we use this to implement polarization-resolved SHG imaging, which allows imaging and identification of structural proteins such as collagen and myosin. We image mouse tail tendon and heart tissue, employing the endoscope at depths up to 1 mm, demonstrating that we can differentiate these structural proteins. This method has the potential for enabling instant and in situ diagnosis of tumors and fibrotic conditions in sensitive tissue with minimal damage. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement