Sf1 and Mis expression: molecular milestones in the canine sex determination pathway.
Sf1 and Mis expression: molecular milestones in the canine sex determination pathway.
复制标题
Sf1 和 Mis 表达：犬性别决定途径的分子里程碑。
DOI：
10.1002/mrd.20217
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Meyers-Wallen,VickiN
中科院分区：
文献类型：
--
作者：
Meyers-Wallen,VickiN
在哺乳动物中，Y - linked基因通常负责睾丸诱导。然而，在sry阴性的XX性逆转的人类患者和动物模型中，睾丸在没有sryin的情况下发育。睾丸诱导这种疾病的机制目前尚不清楚。正常胚胎中基因表达的特征有助于评估犬类阴性XX性别逆转模型的框架。本研究的目的是通过确定sf1和misin正常泌尿生殖嵴（UGR）在不同妊娠阶段的时空表达模式，为犬的性别决定途径增加两个分子里程碑。sf1表达的开始标志着性别决定期的开始，而初始错误表达标志着睾丸诱导期的结束。在卡内基期（CS） 15 ~ 20时，通过定量逆转录-聚合酶链反应（qRT - PCR）和全载原位杂交（WMISH）检测sf1在UGR中的表达。犬的性别决定开始于cs15， sf1在新兴的性腺中表达。gonadalsf1在所有年龄的两性中均有表达，在cs15和cs17时在假定的肾上腺原基中也有表达。≥cs17期时，sf1在雄性和雌性性腺中均有表达。WMISH检测在CS 13.5‐20时，只在≥CS 18的雄性性腺中观察到错误表达，表明睾丸诱导期在CS 18时结束。这两种基因的表达模式与在人类和家畜中观察到的相似。摩尔。天线转换开关。开发与应用，2005:383-389。©2005 Wiley‐Liss, Inc。
In mammals, the Y‐linkedSrygene is normally responsible for testis induction. However, testes develop in the absence ofSryin human patients and animal models withSry‐negative XX sex reversal. The mechanism of testis induction in this disorder is presently unknown. Characterization of gene expression in normal embryos contributes to the framework within which the canineSry‐negative XX sex reversal model can be evaluated. The objective of this study was to add two molecular milestones to the canine sex determination pathway by determining the temporal and spatial expression patterns ofSf1andMisin normal urogenital ridges (UGR) at various gestational stages. The onset ofSf1expression signifies the start of the sex determination period, whereas initialMisexpression identifies the end of the testis induction period.Sf1expression in UGR was measured by quantitative reverse transcription‐polymerase chain reaction (qRT‐PCR) and whole mount in situ hybridization (WMISH) at Carnegie stages (CS) 15 to 20. Canine sex determination begins at CS 15 withSf1expression in the emerging indifferent gonad. GonadalSf1expression was detected in both sexes at all ages, and in the presumptive adrenal primordium at CS 15 and 17. At stages ≥CS 17,Sf1expression was pronounced in male and female gonads.Misexpression, assayed by WMISH at CS 13.5‐20, was observed only in male gonads ≥CS 18, indicating that the testis induction period ends at CS 18. The expression patterns of both genes are similar to those observed in humans and domestic animals. Mol. Reprod. Dev. 70: 383–389, 2005. © 2005 Wiley‐Liss, Inc.