Lowering the cholesterol content of MA104 cells inhibits receptor-mediated transport of folate.
Lowering the cholesterol content of MA104 cells inhibits receptor-mediated transport of folate.
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DOI：
10.1083/jcb.118.1.63
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发表时间：
1992-07
期刊：
影响因子：
--
通讯作者：
Anderson RG
中科院分区：
文献类型：
--
作者：
Chang WJ;Rothberg KG;Kamen BA;Anderson RG
叶酸受体聚集在MA104细胞表面，与小泡相关。这种关系被认为是必要的适当内化和受体的循环过程中5-甲基四氢叶酸输送到叶酸耗尽细胞的细胞质。当细胞被剥夺胆固醇时，受体的集群组织和小泡的完整性都被破坏。我们现在表明，MA104细胞的胆固醇消耗显著降低了5-甲基四氢叶酸内化的速率，并导致内部循环室中存在的受体数量下降70%。这一效应与形态学数据一致，形态学数据显示胆固醇耗尽的MA104细胞的小泡数量减少，每个小泡的受体数量也减少。
The folate receptor is clustered on the surface of MA104 cells in association with caveolae. This relationship is thought to be essential for the proper internalization and recycling of the receptor during the delivery of 5-methyltetrahydrofolate to the cytoplasm of folate- depleted cells. Both the clustered organization of the receptor and the integrity of caveolae are disrupted when cells are deprived of cholesterol. We now show that cholesterol depletion of MA104 cells markedly reduces the rate of 5-methyltetrahydrofolate internalization and causes a 70% decline in the number of receptors present in the internal, recycling compartment. This effect is consistent with morphologic data showing that cholesterol-depleted MA104 cells have a reduced number of caveolae as well as fewer receptors per caveolae.