Sperm deliver a new second messenger: NAADP
Sperm deliver a new second messenger: NAADP
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DOI：
10.1016/s0960-9822(03)00002-2
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发表时间：
2003-01-21
期刊：
影响因子：
9.2
通讯作者：
Galione, A
中科院分区：
文献类型：
--
作者：
Churchill, GC;O'Neill, JS;Galione, A
NAADP 是一种高效的 Ca(2+) 动员剂 [1, 2]，进而在多种物种中触发 Ca(2+) 诱导的 Ca(2+) 释放途径 [3-6] [2, 7]。然而，NAADP 目前并未被归类为第二信使，因为它尚未被证明会随着生理刺激的反应而增加。我们现在报告称，在海胆卵受精过程中，NAADP 急剧增加，这主要是由于接触卵果冻后产生了精子。 NAADP 丸剂在输送到卵子后发挥生理作用，因为它能够诱导皮质闪光，这是一种去极化诱导的 L 型 Ca(2+) 通道激活。此外，在对 NAADP 不敏感的卵子中，精子诱导的皮质闪光被消除。我们得出的结论是，NAADP 的增加在受精过程中发挥着生理相关作用，并首次证明 NAADP 是真正的第二信使。
NAADP is a highly potent mobilizer of Ca(2+) [1, 2], which in turn triggers Ca(2+)-induced Ca(2+) release pathways [3-6] in a wide range of species [2, 7]. Nevertheless, NAADP is not presently classified as a second messenger because it has not been shown to increase in response to a physiological stimulus. We now report a dramatic increase in NAADP during sea urchin egg fertilization that was largely due to production in sperm upon contacting egg jelly. The NAADP bolus plays a physiological role upon delivery to the egg based on its ability to induce a cortical flash, a depolarization-induced activation of L-type Ca(2+) channels. Moreover, the sperm-induced cortical flash was eliminated in eggs desensitized to NAADP. We conclude that an NAADP increase plays a physiologically relevant role during fertilization and provides the first conclusive demonstration that NAADP is a genuine second messenger.