Cytoarchitectonics of substantia nigra grafts: a light and electron microscopic study of immunocytochemically identified dopaminergic neurons and fibrous astrocytes.
Cytoarchitectonics of substantia nigra grafts: a light and electron microscopic study of immunocytochemically identified dopaminergic neurons and fibrous astrocytes.
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黑质移植物的细胞结构：免疫细胞化学鉴定的多巴胺能神经元和纤维星形胶质细胞的光学和电子显微镜研究。
DOI：
10.1002/cne.902310110
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Jaeger,CB
中科院分区：
文献类型：
--
作者：
Jaeger,CB
在大鼠脑中生长长达7个月的黑质移植物中研究了多巴胺能（DA）神经元和星形胶质细胞的成熟。调查有三个具体目标。一是观察不同移植部位对DA能神经元寿命的影响。第二，研究移植物中DA神经元和星形胶质细胞之间的突触相互作用和联系的变化。第三，寻求一个答案的问题是否移植的DA神经元迁移到邻近的宿主脑。移植物取自不同年龄（妊娠14 - 18天）大鼠胚胎的腹侧中脑，并置于新生宿主动物的大脑皮质、顶盖、小脑或脑室中。观察移植物存活不同时间后酪氨酸羟化酶（TH）和胶质丝蛋白（GFA）的免疫细胞化学定位。在所有的移植部位，除了一个，不同形态的神经元，含有TH的移植物中发现。宿主大脑的小脑白色物质不能支持DA神经元的长期存活。成熟的黑质移植物的整体结构与完整的黑质（SN）有一些相似之处。在超微结构水平上，发现DA神经元的一些不成熟特征的形态学表达，如胶质鞘、体细胞棘和少突胶质细胞的缺乏，在成熟移植物中持续存在。DA神经元和星形胶质细胞在移植物中的特定协会表明，一个给定的大脑区域的cytoarchitectonic外观可能与特定的神经元胶质细胞关系的存在。与完整的SN相比，移植显示未标记的多形性终末缺陷，并含有一些TH免疫反应终末。DA能神经元及其突起迁移到邻近宿主脑内的现象很少。
Maturation of dopaminergic (DA) neurons and astroglia was studied in transplants of the substantia nigra grown for up to 7 months in the brain of rats. The investigation had three specific aims. The first was to observe effects of different transplant positions on the longevity of DA neurons. Second, the grafts were examined for changes of synaptic interactions and associations between DA neurons and astroglia. Third, an answer was sought to the question whether transplanted DA neurons migrate into the adjacent host brain. The grafts were taken from the ventral mesencephalon of rat embryos of different ages (day 14 to 18 of gestation) and placed into the cerebral cortex, tectum, cerebellum, or ventricles of newborn host animals. Following different times of survival the immunocytochemical localization of tyrosine hydroxylase (TH) and of glia filament protein (GFA) in the transplants were observed. In all of the transplantation sites, except for one, neurons of different morphologies that contained TH were found in the grafts. The cerebellar white matter of the host brain failed to support the long‐term survival of DA neurons. The overall structure of mature substantia nigra grafts had some resemblance to intact substantia nigra (SN). On the ultrastructural level, it was found that morphological expression of some immature features of DA neurons, such as glial sheaths, somatic spines, and lack of oligodendroglia, persisted in mature grafts. Specific associations of DA neurons and astroglia in the grafts suggested that the cytoarchitectonic appearance of a given brain region may be related to the existence of particular neuron glia relationships. In contrast to intact SN, transplants revealed deficiencies in unlabeled pleomorphic boutons and contained some TH‐immunoreactive terminals. Migration of DA neurons and their processes into the adjacent host brain was rarely observed.