Spermine binding to GC-rich DNA: experimental and theoretical studies.
Spermine binding to GC-rich DNA: experimental and theoretical studies.
复制标题
精胺与富含 GC 的 DNA 结合：实验和理论研究。
DOI：
10.1006/abbi.1996.0005
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发表时间：
1996
影响因子：
3.9
通讯作者：
Haworth,IS
中科院分区：
文献类型：
--
作者：
Yuki,M;Grukhin,V;Lee,CS;Haworth,IS
采用实验和计算方法研究了精胺与DNA的结合。精胺阻断寡核苷酸双链体5 ′-CTTCCAAGATGC ATCAGATG 5′-CATCTGATGC ATCTTGGAAG中2，5-二氮丙啶基苯醌引起的5′-GC链间交联（其中下划线核苷酸碱基代表交联位点）。分子动力学模拟表明，这是精胺优先结合在寡核苷酸的5′-GC大沟位点的结果。使用GC交替序列的进一步模拟显示了对5′-GC步骤的类似偏好。在包括多个精胺分子的模拟中，发生了交替的5′-GC步骤的占据。从这一点，我们推导出一个机制，实验观察到的协同精胺结合聚（dGdC）2。
The DNA binding of spermine has been studied using experimental and computational approaches. Spermine blocks 5′-GC interstrand crosslinking by 2,5-diaziridinylbenzoquinone in the oligonucleotide duplex 5′-CTTCCAAGATGC ATCAGATG 5′-CATCTGATGC ATCTTGGAAG (where the underlined nucleotide bases represent the crosslinking site). Molecular dynamics simulations suggest that this is a result of preferential spermine binding at the 5′-GC major groove site of the oligonucleotide. A further simulation with a GC-alternating sequence shows a similar preference for the 5′-GC step. In a simulation including multiple spermine molecules, occupation of alternate 5′-GC steps occurred. From this, we deduce a mechanism for the experimentally observed cooperativity of spermine binding to poly(dGdC)2.