Neuromodulatory role of acetylcholine in visually-induced cortical activation: Behavioral and neuroanatomical correlates
Neuromodulatory role of acetylcholine in visually-induced cortical activation: Behavioral and neuroanatomical correlates
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DOI：
10.1016/j.neuroscience.2008.04.030
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发表时间：
2008-07-17
期刊：
影响因子：
3.3
通讯作者：
Vaucher, E.
中科院分区：
文献类型：
--
作者：
Dotigny, F.;Ben Amor, A. Y.;Vaucher, E.
乙酰胆碱在视觉刺激过程中在初级视皮层中释放，并且可能在视觉处理中具有神经调节作用。本研究使用行为和功能神经解剖学的调查，以检查在大鼠的这一作用。在第一组实验中，用192免疫球蛋白G（IgG）皂草素损伤胆碱能系统，并评估视觉水迷宫任务中的视觉敏锐度和性能。胆碱能损伤并不影响视力测量前和损伤后，但它确实降低了效率，学习一种新的方向辨别任务测量损伤后。为了更好地理解胆碱能系统参与视觉皮层神经元活动，进一步研究了图案化视觉刺激诱导的c-Fos表达。将胆碱能纤维损伤（192 IgG-皂草素或使君子酸）、毒蕈碱抑制（东莨菪碱）或NMDA受体抑制（CPP）后获得的结果与对照条件进行比较。使用双重和三重免疫标记以确定激活的皮质细胞的神经化学性质。结果表明，图形刺激引起对侧初级视皮层第IV层c-Fos免疫标记神经元显著增加，胆碱能纤维损伤和东莨菪碱可完全抑制图形刺激引起的c-Fos免疫标记神经元的增加。这种作用不依赖于NMDA受体的传递。c-Fos的激活主要在海马能棘状星状细胞中观察到，而在GABA能中间神经元中较少。总之，这些结果表明，强烈参与的基底前脑胆碱能系统的调制突触后视觉处理，这可能与认知增强或注意力在视觉学习。(C)2008年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Acetylcholine is released in the primary visual cortex during visual stimulation and may have a neuromodulatory role in visual processing. The present study uses both behavioral and functional neuroanatomy investigations to examine this role in the rat. In the first set of experiments the cholinergic system was lesioned with 192 immunoglobulin G (IgG) saporin and the visual acuity and performance in a visual water maze task were assessed. The cholinergic lesion did not affect the visual acuity measured pre- and post-lesion but it did reduce the efficiency to learn a novel orientation discrimination task measured post-lesion. In order to better understand the involvement of the cholinergic system in the neuronal activity in the visual cortex c-Fos expression induced by patterned visual stimulation was further investigated. Results obtained following lesion of the cholinergic fibers (192 IgG-saporin or quisqualic acid), muscarinic inhibition (scopolamine), or NMDA receptor inhibition (CPP) were compared with control conditions. Double and triple immunolabeling was used in order to determine the neurochemical nature of the activated cortical cells. The results demonstrated that patterned stimulation elicited a significant increase in c-Fos immunolabeled neurons in layer IV of the contralateral primary visual cortex to the stimulated eye which was completely abolished by cholinergic fibers lesion as well as scopolamine administration. This effect was independent of NMDA receptor transmission. The c-Fos activation was predominantly observed in the glutamatergic spiny stellate cells and less frequently in GABAergic interneurons. Altogether, these results demonstrate a strong involvement of the basal forebrain cholinergic system in the modulation of post-synaptic visual processing, which could be related to cognitive enhancement or attention during visual learning. (C) 2008 IBRO. Published by Elsevier Ltd. All rights reserved.