Role of the spleen in liver fibrosis in rats may be mediated by transforming growth factor β-1
Role of the spleen in liver fibrosis in rats may be mediated by transforming growth factor β-1
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DOI：
10.1046/j.1440-1746.2002.02667.x
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发表时间：
2002-01-01
影响因子：
4.1
通讯作者：
Sugimachi, K
中科院分区：
文献类型：
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作者：
Akahoshi, T;Hashizume, M;Sugimachi, K
背景：脾脏对肝病的影响尚不清楚。转化生长因子-β 1（Transforming growth factor-beta1，TGF-β 1）是肝细胞再生的抑制因子，在肝纤维化的基质生成中起重要作用。在这项研究中，我们调查了TGF-β 1的生产在脾脏的asthma大鼠和脾切除术的愈合过程中的肝纤维化的影响。硫代乙酰胺（TAA）腹腔注射24周。大鼠进行假手术（TAA+Sham）或脾切除术（TAA+SPL）。观察各组术后10、30、60 d肝纤维化和肝再生的改善情况。通过ELISA研究脾切除术对门静脉中TGF-β 1血浆浓度的影响。采用免疫组织化学染色法检测脾脏中TGF-β 1的表达，并采用RT-PCR法检测其表达程度。结果：TAA+SPL组大鼠肝再生（PCNA标记指数）在术后10、30 d明显高于对照组（P < 0. 05），而TAA+SPL组大鼠肝再生（PCNA标记指数）在术后10、30 d明显高于对照组（P < 0. 05）。
Background: The effect of the spleen on the cirrhotic liver is unknown. Transforming growth factor-beta1 (TGF-beta1), which plays a crucial role in the matrix production during liver fibrosis, is an inhibitory factor regarding the regeneration of hepatocytes. In this study, we investigated the TGF-beta1 production in the spleen of cirrhotic rats and the effects of a splenectomy on the healing process from liver fibrosis.Methods: Thirty-six Wister male rats were used. Thioacetamide (TAA) was administered intraperitoneally for 24 weeks. The rats underwent either a sham operation (TAA+Sham) or a splenectomy (TAA+SPL). The improvements in liver fibrosis and liver regeneration were investigated 10, 30 and 60 days after the operations in each group. The effect of a splenectomy on the plasma concentration of TGF-beta1 in the portal vein was investigated by ELISA. The TGF-beta1 expressions in the spleen were measured using immunohistochemical staining and the degree of such expression was measured using RT-PCR. The activity of TGF-beta1 in the portal vein of TAA + Sham and TAA + SPL was assessed by the inhibiting effect of rat parenchymal hepatocyte proliferation in primary culture.Results: Liver regeneration (PCNA-labeling index) in the TAA+SPL rats was stimulated more at 10 and 30 days after the operation (P