Cosmid-derived transcripts and sequence tags mapped to three subregions of human chromosome 22.
Cosmid-derived transcripts and sequence tags mapped to three subregions of human chromosome 22.
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粘粒衍生的转录物和序列标签映射到人类 22 号染色体的三个子区域。
DOI：
10.1016/s0378-1119(96)00411-8
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发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
Blin,N
中科院分区：
文献类型：
--
作者：
Pusch,C;Müllenbach,R;Gött,P;Schmitt,H;Wang,Z;Roe,B;Blin,N
从伦敦ICRF和加利福尼亚劳伦斯利弗莫尔实验室的人类22号染色体cosmid文库中分离出50个cosmid，进行区域分配并测试其检测重复或单拷贝序列的能力。搜索结果显示，9个cosmids包含来自22号染色体中心周围区域的重复基序。另外19个cosmids在22q11.2-q13.1和22q13.1-qter区域的22q: 7染色体长臂上检测到单拷贝序列，并通过荧光原位杂交更精确地定位。这19个重组体中有3个显示含有（CA）nrepeats的限制性内切片段，并进行了亚克隆和测序。一个cosmid，代表了一个无处不在的300 bp转录本的编码区域，位于PDGFB 600 kb处，四个cosmid包含了ARSA基因22q13.3周围的序列。目前，人们正在利用现有的cosmid和新的细菌人工染色体（BAC）文库，从22q13染色体上附加的区域分配标记构建远程物理图谱，这可能有助于分析22q13染色体的结构变化。
Fifty cosmids from the ICRF, London, and Lawrence Livermore Laboratory, California, human chromosome 22 cosmid libraries were isolated, regionally assigned and tested for their ability to detect repeats or single copy sequences. The search resulted in nine cosmids containing repetitive motifs from the pericentric region of chromosome 22. An additional 19 cosmids, that detected single copy sequences in the long arm of chromosome 22q: 7 in the region 22q11.2-q13.1 and 12 in 22q13.1-qter, were mapped more precisely by fluorescence in situ hybridization. Three out of these 19 recombinants displayed restriction fragments containing (CA)nrepeats, were subcloned and sequenced. One cosmid, representing a region coding for an ubiquitous 300-bp transcript, is localized 600 kb from PDGFB, and four cosmids contained sequences surrounding the ARSA gene at 22q13.3. Presently, long range physical maps, that may be useful for analysing structural alterations of chromosome 22q13, are being constructed from these additional, regionally assigned markers from chromosome 22q13 employing both existing cosmid and new bacterial artificial chromosome (BAC) libraries.