Arcuate nucleus of hypothalamus is involved in mediating the satiety effect of electroacupuncture in obese rats
Arcuate nucleus of hypothalamus is involved in mediating the satiety effect of electroacupuncture in obese rats
复制标题
下丘脑弓状核参与介导肥胖大鼠电针的饱腹感作用
DOI：
10.1016/j.peptides.2011.10.019
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发表时间：
2011-12-01
期刊：
影响因子：
3
通讯作者：
Han, Ji Sheng
中科院分区：
文献类型：
--
作者：
Wang, Fei;Tian, De Run;Han, Ji Sheng
肥胖是世界上一个主要的健康问题。由于有效的治疗方法很少见，研究人员正试图发现治疗肥胖的新方法，而针灸是最受欢迎的替代方法之一。本研究采用饮食诱导的肥胖大鼠模型，探讨电针的减肥作用机制。在用高脂肪饮食喂养9周后，体重增加超过饲料喂养组大鼠最大体重的许多大鼠被认为是肥胖的，并用于研究。电针治疗组大鼠每日1次，每次2 Hz，电针强度从0.5-1- 1.5mA递增，每次30 min，与DIO组和束缚组相比，电针组大鼠摄食量明显减少，体重明显减轻。电针处理增加了下丘脑弓状核（ARH）神经元中α-MSH的肽水平及其前体POMC的mRNA水平。此外，脑脊髓液（CSF）中的α-MSH的含量通过EA应用而升高。谷氨酸损毁ARH后，电针对大鼠摄食量和体重的抑制作用消失。与束缚和ST 36/SP 6电针治疗相比，非穴位刺激没有显示出对摄食抑制和体重减轻的有益效果。我们的结论是，在ST 36/SP 6 EA治疗通过ARH显着抑制摄食量和体重增加时，喂养高脂饮食和刺激α-MSH的表达和释放可能参与的机制。(C)2011 Elsevier Inc. All rights reserved.
Obesity is a major health problem in the world. Since effective remedies are rare, researchers are trying to discover new therapies for obesity, and acupuncture is among the most popular alternative approaches. This study investigated the anti-obesity mechanisms of EA, using a rat model of diet-induced obesity. After feeding with a high-fat diet for 9 weeks, a number of rats who gained weight that surpassed the maximal body weight of rats in the chow-fed group were considered obese and employed in the study. A 2 Hz EA treatment at the acupoints ST36/SP6 with the intensity increasing stepwise from 0.5-1-1.5 mA was given once a day for 30 min. Rats treated with EA showed significantly decreased food intake and reduced body weight compared with the rats in DIO and restraint group. EA treatment increased peptide levels of alpha-MSH and mRNA levels of its precursor POMC in the arcuate nuclear of hypothalamus (ARH) neurons. In addition, the cerebral spinal fluid (CSF) content of alpha-MSH was elevated by EA application. ARH lesions by monosodium glutamate abolished the inhibition effect of EA on food intake and body weight. A non-acupoint stimulation did not show the benefit effect on food intake inhibition and body weight reduction compared with restraint and ST36/SP6 EA treatment. We concluded that EA treatment at ST36/SP6 acted through ARH to significantly inhibit food intake and body weight gain when fed a high-fat diet and that the stimulation of alpha-MSH expression and release might be involved in the mechanism. (C) 2011 Elsevier Inc. All rights reserved.