Distribution of tracheal and laryngeal mucous glands in some rodents and the rabbit.
Distribution of tracheal and laryngeal mucous glands in some rodents and the rabbit.
复制标题
一些啮齿动物和兔子的气管和喉粘液腺的分布。
DOI：
10.1046/j.1469-7580.2001.19820207.x
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发表时间：
2001
影响因子：
2.4
通讯作者：
Bastacky,SJ
中科院分区：
文献类型：
--
作者：
Widdicombe,JH;Chen,LL;Sporer,H;Choi,HK;Pecson,IS;Bastacky,SJ
我们用扫描电镜对大鼠、豚鼠、仓鼠、小鼠和家兔的气管和喉部的粘液腺开口数量进行了计数。通过蛋白酶消化去除气道表面上皮细胞，更好地观察腺体开口。采用常规组织学和PAS/阿利新蓝染色法进一步研究腺体分布。在所有啮齿类动物中，喉部腺体开口的发生频率为每平方毫米1-2个。老鼠的气管中没有腺体开口，仓鼠只有少数腺体开口。大鼠气管含有126±42个腺体开口（±sd; n = 6），气管顶部的频率为每mm2 ~ 0.6个，底部的频率为每mm2 ~ 0.15个。豚鼠气管有153±90个腺体开口（±sd; n = 5），其中54%位于气管的前40%。在大鼠和豚鼠中，在软骨环之间的腹侧发现了气管腺，而在背膜部分则没有。大多数物种的腺体开口是直径约50 μm的简单圆形。然而，大鼠气管中的腺体通常斜开成浅的（~ 20 μm深）椭圆形凹槽（~ 150×75 μm），其长轴从头到尾定向。在家兔中，没有气管或喉腺的组织学证据。然而，气管和喉部表面包含许多凹坑（直径约30 μm），以每mm2约4个的频率均匀分布在软骨上和软骨之间。这些可能与其他人描述的杯状细胞的“巢”相对应。
We used scanning electron microscopy to count the number of mucous gland openings in the tracheae and lower portion of the larynges of the rat, guinea pig, hamster, mouse and rabbit. Cells of the airway surface epithelium were removed by protease digestion better to visualise the gland openings. The distribution of glands was further studied by conventional histology and by PAS/Alcian blue staining of whole mounts. In all rodent species, gland openings in the larynx occurred with a frequency of 1–2 per mm2. Mice had no gland openings in their tracheae, and hamsters, only a handful. Rat tracheae contained 126±42 gland openings (±S.D.; n = 6) at a frequency of ∼ 0.6 per mm2 at the top of the trachea and ∼ 0.15 per mm2 at the bottom. Guinea pig tracheae contained 153±90 gland openings (±S.D.; n = 5), with 54% being in the top 40% of the trachea. In both rat and guinea pig, tracheal glands were found in the ventral aspect between the cartilaginous rings, and were absent from the dorsal membranous portion. Gland openings in most species were simple circles of ∼ 50 μm diameter. However, glands in the rat trachea generally opened obliquely into shallow (∼ 20 μm deep) oval troughs (∼ 150×75 μm), which had their long axes oriented from head to tail. In the rabbit, there was no evidence of tracheal or laryngeal glands histologically. However, the tracheal and laryngeal surfaces contained numerous pits (∼ 30 μm diameter) distributed evenly over and between cartilages at a frequency of ∼ 4 per mm2. These may correspond to the ‘nests’ of goblet cells described by others.