Complementary profiling of gene expression at the transcriptome and proteome levels in Saccharomyces cerevisiae
Complementary profiling of gene expression at the transcriptome and proteome levels in Saccharomyces cerevisiae
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DOI：
10.1074/mcp.m200001-mcp200
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发表时间：
2002-04-01
影响因子：
7
通讯作者：
Aebersold, R
中科院分区：
文献类型：
--
作者：
Griffin, TJ;Gygi, SP;Aebersold, R
使用一个集成的基因组和蛋白质组学的方法，我们已经研究了碳源扰动对半乳糖或乙醇上生长的酿酒酵母中稳态基因表达的影响。对于许多基因，观察到信使RNA转录物和相应蛋白质产物的丰度比之间的显着差异。对呼吸、能量产生和蛋白质合成相关基因的微扰效应的深入了解，从单独在信使RNA或蛋白质水平上进行的测量中是不明显的，这说明了整合从同一样品中获得的不同类型数据的能力，用于生物系统和过程的综合表征。
Using an integrated genomic and proteomic approach, we have investigated the effects of carbon source perturbation on steady-state gene expression in the yeast Saccharomyces cerevisiae growing on either galactose or ethanol. For many genes, significant differences between the abundance ratio of the messenger RNA transcript and the corresponding protein product were observed. Insights into the perturbative effects on genes involved in respiration, energy generation, and protein synthesis were obtained that would not have been apparent from measurements made at either the messenger RNA or protein level alone, illustrating the power of integrating different types of data obtained from the same sample for the comprehensive characterization of biological systems and processes.