EFFECT OF BRADYKININ AND THROMBIN ON PROSTACYCLIN SYNTHESIS IN ENDOTHELIAL-CELLS FROM CALF AND PIG AORTA AND HUMAN UMBILICAL-CORD VEIN
EFFECT OF BRADYKININ AND THROMBIN ON PROSTACYCLIN SYNTHESIS IN ENDOTHELIAL-CELLS FROM CALF AND PIG AORTA AND HUMAN UMBILICAL-CORD VEIN
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DOI：
10.1016/0049-3848(80)90201-7
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发表时间：
1980-01-01
影响因子：
7.5
通讯作者：
HONG, SL
中科院分区：
文献类型：
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作者：
HONG, SL
缓激肽可使牛、猪主动脉和人脐静脉内皮细胞合成前列环素。在10 ng缓激肽/ml时达到最大刺激，PG[前列腺素]I2增加10倍，如通过6-酮-PGF 1 α的放射免疫测定所测定的。离子载体A23187 [钙霉素]、胰蛋白酶和前体花生四烯酸也具有刺激性。凝血酶对人脐静脉内皮细胞合成PGI_2的影响来自小牛或猪主动脉的细胞也不受凝血酶刺激。这些细胞对凝血酶的反应似乎存在差异。当小牛细胞在[3 H]花生四烯酸存在下生长时，放射性主要掺入到磷脂中。缓激肽，离子载体A23187和胰蛋白酶刺激释放放射性物质进入介质从[3 H]花生四烯酸标记的小牛细胞。花生四烯酸是释放的主要放射性物质，PGI 2是形成的主要已知花生四烯酸代谢物。
Bradykinin causes the synthesis of prostacyclin in endothelial cells cultured from calf and pig aorta and human umbilical cord vein. Maximal stimulation is attained at 10 ng bradykinin per ml with a 10-fold increase of PG[prostaglandin]I2 as assayed by radioimmunoassay for 6-keto-PGF1.alpha.. Ionophore A23187 [calcimycin], trypsin and the precursor arachidonic acid are also stimulatory. Thrombin is effective in stimulating PGI2 synthesis in endothelial cells from human umbilical cord vein. Cells from calf or pig aorta are not stimulated as well by thrombin. There appears to be a difference in the response to thrombin between these cells. When calf cells were grown in the presence of [3H]arachidonic acid the radioactivity is incorporated mainly into the phospholipids. Bradykinin, ionophore A23187 and trypsin stimulated the release of radioactive materials into medium from [3H]arachidonic acid-labeled calf cells. Arachidonic acid is the major radioactive substance released and PGI2 is the major known arachidonic acid metabolite formed.