Cell body shape and directional movement stability in human-induced pluripotent stem cell-derived dopaminergic neurons
Cell body shape and directional movement stability in human-induced pluripotent stem cell-derived dopaminergic neurons
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DOI：
10.1038/s41598-020-62598-4
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发表时间：
2020-04-02
影响因子：
4.6
通讯作者：
Ozaki, Norio
中科院分区：
文献类型：
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作者：
Arioka, Yuko;Shishido, Emiko;Ozaki, Norio
神经元迁移在脑结构的形成过程中是必需的。最近，我们在体外证实了人诱导多能干细胞(IPSC)来源的多巴胺能神经元表现出定向迁移。然而，目前尚不清楚细胞形状如何参与它们的迁移。在这项研究中，我们使用人iPSC来源的多巴胺能神经元进行了实时成像分析。我们的自动化方法可以自动识别特定时间点的细胞体形状和细胞位置，揭示了健康的IPSC来源的多巴胺能神经元根据其形状迁移。这种迁移行为在携带RELN缺失的IPSCs来源的神经元中是不一致的。我们的发现提供了一种新的理论，即在RELN的调控下，细胞体的取向与人多巴胺能神经元运动方向的稳定性有关。
Neuronal migration is necessary in the process of the formation of brain architecture. Recently, we demonstrated that human induced pluripotent stem cell (iPSC)-derived dopaminergic neurons exhibit directional migration in vitro. However, it remains unclear how the cell shape is involved in their migration. In this study, we performed live imaging analyses using human iPSC-derived dopaminergic neurons. Our automated method, which can automatically identify the cell body shape and the cell position at specific time points, revealed that healthy iPSC-derived dopaminergic neurons migrate according to their shape. This migration behavior was out of accord in neurons derived from iPSCs carrying an RELN deletion. Our findings provide a novel theory that cell body orientation is related to the stability of movement direction for human dopaminergic neurons, under the regulation of RELN.