Diversity and Composition of Bacterial Community in Soils and Lake Sediments from an Arctic Lake Area.
Diversity and Composition of Bacterial Community in Soils and Lake Sediments from an Arctic Lake Area.
复制标题
DOI：
10.3389/fmicb.2016.01170
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Zang JY
中科院分区：
文献类型：
--
作者：
Wang NF;Zhang T;Yang X;Wang S;Yu Y;Dong LL;Guo YD;Ma YX;Zang JY
本研究评估了北极湖区（斯瓦尔巴群岛伦敦岛）土壤和湖泊沉积物中细菌群落的多样性和组成。以细菌16 S rRNA基因为靶基因，通过高通量测序共鉴定了2,987个操作分类单位。4个采样点（每个采样点3个样品）的地球化学性质和细菌群落组成存在明显差异。9个土壤样品中变形菌和酸性细菌是丰富的门，而3个沉积物样品中变形菌和拟杆菌是丰富的门。此外，放线菌、绿菌、绿弯菌、Elusimicrobia、厚壁菌门、芽单胞菌、硝化螺旋菌、浮游菌、变形菌在4个采样点的丰度差异显著。此外，优势属的成员，如梭菌，Luteolibacter，Methylibium，红球菌和Rhodoplanes，在它们的丰度之间的4个采样点显着差异。此外，基于距离的冗余度分析表明，pH（p < 0.001），含水量（p < 0.01），铵态氮（-N，p < 0.01），硅酸盐硅（-Si，p < 0.01），亚硝酸盐氮（-N，p < 0.05）、有机碳（p < 0.05）和有机氮（p < 0.05）是与细菌群落组成相关性最显著的因子。研究结果表明，北极某湖区土壤和沉积物中细菌群落具有较高的多样性，其多样性受北极环境的多种地球化学因素的影响。
This study assessed the diversity and composition of bacterial communities within soils and lake sediments from an Arctic lake area (London Island, Svalbard). A total of 2,987 operational taxonomic units were identified by high-throughput sequencing, targeting bacterial 16S rRNA gene. The samples from four sites (three samples in each site) were significantly different in geochemical properties and bacterial community composition. Proteobacteria and Acidobacteria were abundant phyla in the nine soil samples, whereas Proteobacteria and Bacteroidetes were abundant phyla in the three sediment samples. Furthermore, Actinobacteria, Chlorobi, Chloroflexi, Elusimicrobia, Firmicutes, Gemmatimonadetes, Nitrospirae, Planctomycetes, Proteobacteria significantly varied in their abundance among the four sampling sites. Additionally, members of the dominant genera, such as Clostridium, Luteolibacter, Methylibium, Rhodococcus, and Rhodoplanes, were significantly different in their abundance among the four sampling sites. Besides, distance-based redundancy analysis revealed that pH (p < 0.001), water content (p < 0.01), ammonium nitrogen (-N, p < 0.01), silicate silicon (-Si, p < 0.01), nitrite nitrogen (-N, p < 0.05), organic carbon (p < 0.05), and organic nitrogen (p < 0.05) were the most significant factors that correlated with the bacterial community composition. The results suggest soils and sediments from a lake area in the Arctic harbor a high diversity of bacterial communities, which are influenced by many geochemical factors of Arctic environments.