Immunohistochemical mapping of natriuretic peptide receptor-A in the brainstem of Macaca fascicularis
Immunohistochemical mapping of natriuretic peptide receptor-A in the brainstem of Macaca fascicularis
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DOI：
10.1016/j.neuroscience.2006.12.062
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发表时间：
2007-03-30
期刊：
影响因子：
3.3
通讯作者：
Tooyama, I.
中科院分区：
文献类型：
--
作者：
Abdelalim, E. M.;Osman, A. H. K.;Tooyama, I.
钠尿肽受体A（NPR-A）介导心钠素（ANP）和脑钠素（BNP）的生物学效应，参与维持心血管稳态。在这项免疫组织化学研究中，我们研究了NPR-A在食蟹猴脑干中的分布。NPR-A免疫反应定位于特定脑干区域的神经元。NPR-A免疫反应阳性核体分布于中脑红核和眼神经核，脑桥臂旁核和蓝斑，延髓迷走神经背核、舌下神经核、楔束核、薄束核、疑核、外侧网状核、网状结构和下橄榄核。广泛的免疫反应纤维网络在红核、眼神经核、三叉神经感觉主核和臂旁核中是明显的。双标免疫组化结果显示，NPR-A免疫反应阳性的胆碱能神经元的臂旁核，迷走神经背侧运动核，舌下神经核，疑核。然而，在蓝斑中没有NPR-A和酪氨酸羟化酶的共定位。NPR-A-免疫反应结构的广泛解剖分布表明，利钠肽，除了具有内分泌和心血管稳态的中枢调节作用，也可以介导不同的生理功能。(c)2007年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Natriuretic peptide receptor-A (NPR-A) mediates the biological effects of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), and is involved in maintaining cardiovascular homeostasis. In this immunohistochemical study we examined the distribution of NPR-A in the brainstem of the cynomoigus monkey. NPR-A immunoreactivity was localized to neurons in specific brainstem regions. NPR-A-immunoreactive perikarya were found in the red nucleus and the oculomotor nucleus in the midbrain, the parabrachial nucleus and the locus coeruleus in the pons, and the dorsal motor nucleus of the vagus, the hypoglossal nucleus, the cuneate nucleus, the gracile nucleus, the nucleus ambiguus, the lateral reticular nucleus, the reticular formation, and the inferior olivary nucleus in the medulla oblongata. Extensive networks of immunoreactive fibers were apparent in the red nucleus, the oculomotor nucleus, the principal sensory trigeminal nucleus, and the parabrachial nucleus. Double immunostaining revealed NPR-A immunoreactivity in cholinergic neurons of the parabrachial nucleus, the dorsal motor nucleus of vagus, the hypoglossal nucleus, and the nucleus ambiguus. However, there was no colocalization of NPR-A and tyrosine hydroxylase in the locus coeruleus. The wide anatomical distribution of NPR-A-immunoreactive structures suggests that natriuretic peptides, besides having a role in the central regulation of endocrine and cardiovascular homeostasis, may also mediate diverse physiological functions. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.