Detection of chromosome aneuploidy in interphase nuclei from human primary breast tumors using chromosome-specific repetitive DNA probes.
Detection of chromosome aneuploidy in interphase nuclei from human primary breast tumors using chromosome-specific repetitive DNA probes.
复制标题
使用染色体特异性重复 DNA 探针检测人类原发性乳腺肿瘤间期细胞核中的染色体非整倍性。
作者：
P. Devilee;R. F. Thierry;T. Kievits;R. Kolluri;A. Hopman;H. Willard;P. Pearson;C. Cornelisse
我们使用染色体特异的重复DNA序列的原位杂交作为探针，以揭示特定的染色体在间期核中作为不同的信号点或信号簇。使用核型定义的细胞和细胞系，我们表明每个核获得的信号数量与该核中存在的特定染色体的数量相关。此外，还可以检测到核型不同的细胞系的混合物。乳腺癌细胞系MCF-7的细胞核和中期分裂相的原位杂交显示，异常数目的点/核表明染色体数目和/或结构异常。在所研究的7个原发乳腺肿瘤中，我们检测到了1号和/或18号染色体靶点的数量异常。虽然所有病例在DNA流动测量中都是单峰，但其中6例在斑点/核含量方面似乎是不同的。
We have used in situ hybridization with chromosome specific repetitive DNA sequences as a probe to reveal particular chromosomes as distinct spots or clusters of signal within interphase nuclei. Using karyotypically defined cells and cell lines, we show that the number of signals obtained per nucleus correlates with the number of particular chromosomes present in that nucleus. Further, admixtures of karyotypically different cell lines could be detected. In situ hybridization of nuclei and metaphase spreads derived from the breast cancer cell line MCF-7 shows that a deviant number of spots/nucleus indicates a numerical and/or structural chromosomal aberration. In seven primary breast tumors studied, we detected numerical aberrations of the target sites of chromosomes 1 and/or 18. Although all had a single peak in DNA flow measurements, six of the cases appeared to be heterogeneous with respect to their spots/nucleus content.
DOI：
10.1073/pnas.78.11.6633
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
LANGER, PR;WALDROP, AA;WARD, DC
通讯作者：
WARD, DC
影响因子：
3.8
作者：
Tewes, Mitra;Aktas, Bahriye;Kasimir-Bauer, Sabine
通讯作者：
Kasimir-Bauer, Sabine
实体瘤及其他肿瘤中的染色体。
影响因子：
11.2
作者：
Sandberg,AA;Turc-Carel,C;Gemmill,RM
通讯作者：
Gemmill,RM