The metabolic profile elucidation of Lonicera japonica flos water extract and the metabolic characteristics evaluation of bioactive compounds in human gastrointestinal tract in vitro
The metabolic profile elucidation of Lonicera japonica flos water extract and the metabolic characteristics evaluation of bioactive compounds in human gastrointestinal tract in vitro
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DOI：
10.1016/j.jpba.2022.114906
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发表时间：
2022-09-20
影响因子：
3.4
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Chang, Yanli;Li, Caixia;Liu, Bin
金银花（Lonicera japonica Flos， LJF）在中国作为保健食品和药用植物长期口服。采用胃液、肠液和人肠道菌群三种独立模型，采用UPLC-LTQ-Orbitrap-MSn进行定性分析，HPLC-DAD进行定量分析。从LJF水提液（LJF- we）中共检出72种原型化合物，包括14种有机酸、43种环烯醚萜、14种黄酮类化合物和1种其他化合物。通过模拟胃液（70和12）、肠液（69和12）和人类粪便细菌（29和70），分别对LJF-WE的原型和代谢成分进行了表征。代谢产物经过脱糖、异构化、氢化、甲基化、脱水、环化、葡萄糖醛酸化和二甲基化形成。与人类粪便细菌相比，新绿原酸、绿原酸、隐绿原酸、苦参苷、刺草苷、异绿原酸B、异绿原酸A和异绿原酸C等8种生物活性化合物在模拟胃液和肠液中具有较好的稳定性。特别是，由于人类粪便细菌中丰富的-葡萄糖苷酶，带有葡萄糖苷键的甜菊苷和塞克罗马苷降解得非常快。
Lonicera japonica Flos (LJF) is taken orally as a health food and medicinal plant in China for a long time. The gastrointestinal metabolism of LJF was investigated in vitro by three independent models (gastric juice, intestinal juice, and human intestinal bacteria), qualitative analyzed by UPLC-LTQ-Orbitrap-MSn and quantified by HPLC-DAD. 72 prototype compounds were detected in LJF water extraction (LJF-WE), including 14 organic acids, 43 iridoids, 14 flavonoids and one other compound. The prototype and metabolic components of LJF-WE bio-transformed by simulated gastric fluid (70 and 12), intestinal fluid (69 and 12) and human fecal bacteria (29 and 70) were characterized, respectively. The metabolites were formed through desaccharization, isomerization, hydrogenation, methylation, dehydration, and then cyclization, glucuronization and dimethylation followed. 8 bioactive compounds including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, sweroside, secoxyloganin, isochlorogenic acid B, isochlorogenic acid A and isochlorogenic acid C were much stable in simulated gastric fluid and intestinal fluid, compared with human fecal bacteria. Especially, sweroside and secoxyloganin with glucoside bonds degradated extraordinarily fast, because of the abundant beta-glucosidases in human fecal bacteria.